PMID- 9329642
OWN - NLM
STAT- MEDLINE
DCOM- 19971103
LR  - 20191024
IS  - 0893-6692 (Print)
IS  - 0893-6692 (Linking)
VI  - 30
IP  - 2
DP  - 1997
TI  - Use of fluorescence in situ hybridization (FISH) to assess effects of smoking, 
      caffeine, and alcohol on aneuploidy load in sperm of healthy men.
PG  - 175-83
AB  - Aneuploidy is a common cause of poor reproductive outcomes in humans and is 
      associated with severe medical problems in liveborn offspring, yet little is 
      known about its underlying cause. A substantial amount of aneuploidy is known to 
      be contributed by the father through cytogenetically abnormal sperm. The purpose 
      of this cross-sectional, observational study was to investigate the potential 
      contribution of common lifestyle exposures (smoking, caffeine, and alcohol) to 
      the aneuploidy load in sperm from 45 healthy male volunteers 19-35 years of age. 
      Sperm FISH (fluorescence in situ hybridization) was used to determine aneuploidy 
      and diploidy frequencies for chromosomes X, Y and 18 across varying exposure 
      levels of smoking, caffeine, and alcohol. Caffeine was significantly associated 
      with increased frequencies of sperm aneuploidy XX18 and XY18, diploidy XY18-18 
      and the duplication phenotype YY18-18 controlling for alcohol, smoking and donor 
      age. Alcohol was significantly associated with increased frequencies of sperm 
      aneuploidy XX18, diploidy XY18-18 and the duplication phenotype XX18-18 
      controlling for caffeine, smoking and donor age. There was a suggestive, but 
      unstable, association between smoking and XX18. Even within our truncated age 
      range, we were able to confirm an increased risk for XX18 aneuploidy with 
      increasing donor age. Sperm FISH proved to be a useful biomarker to detect and 
      compare numerical cytogenetic abnormalities in human sperm cells across differing 
      levels of exposure to smoking, caffeine, and alcohol.
FAU - Robbins, W A
AU  - Robbins WA
AD  - School of Public Health, University of North Carolina at Chapel Hill 27599-7400, 
      USA. robbins2@niehs.nih.gov
FAU - Vine, M F
AU  - Vine MF
FAU - Truong, K Y
AU  - Truong KY
FAU - Everson, R B
AU  - Everson RB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Environ Mol Mutagen
JT  - Environmental and molecular mutagenesis
JID - 8800109
RN  - 3G6A5W338E (Caffeine)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - *Alcohol Drinking
MH  - Aneuploidy
MH  - Caffeine/*pharmacology
MH  - Cross-Sectional Studies
MH  - Diploidy
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Smoking/*adverse effects
MH  - Spermatozoa/*drug effects
EDAT- 1997/01/01 00:00
MHDA- 1997/11/04 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/11/04 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - 10.1002/(SICI)1098-2280(1997)30:2<175::AID-EM10>3.0.CO;2-A [pii]
AID - 10.1002/(sici)1098-2280(1997)30:2<175::aid-em10>3.0.co;2-a [doi]
PST - ppublish
SO  - Environ Mol Mutagen. 1997;30(2):175-83. doi: 
      10.1002/(sici)1098-2280(1997)30:2<175::aid-em10>3.0.co;2-a.