PMID- 9330615
OWN - NLM
STAT- MEDLINE
DCOM- 19971027
LR  - 20190702
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 384
IP  - 3
DP  - 1997 Sep
TI  - Characterization of a human homolog of (6-4) photolyase.
PG  - 195-204
AB  - (6-4)Photolyase catalyzes light-dependent repair of UV-induced pyrimidine (6-4) 
      pyrimidone photoproducts. A human cDNA clone which has high sequence homology to 
      the (6-4)photolyase gene (H64PRH gene) was identified. In this paper we also 
      isolated a genomic clone corresponding to the H64PRH cDNA and mapped it to 
      chromosome 12q24.1 by fluorescence in situ hybridization (FISH). Northern-blot 
      analysis revealed transcription of this gene in all human tissues examined. The 
      H64PRH protein was overproduced in E. coli, partially purified and characterized. 
      Like (6-4)photolyase, the enzyme contains two chromophores, one of which is FAD. 
      However, the enzyme does not show any detectable photolyase activity.
FAU - Todo, T
AU  - Todo T
AD  - Radiation Biology Center, Kyoto University, Japan. Todo@house.rbc.kyoto-u.ac.jp
FAU - Tsuji, H
AU  - Tsuji H
FAU - Otoshi, E
AU  - Otoshi E
FAU - Hitomi, K
AU  - Hitomi K
FAU - Kim, S T
AU  - Kim ST
FAU - Ikenaga, M
AU  - Ikenaga M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 9007-49-2 (DNA)
RN  - EC 4.1.99.- (pyrimidine(6-4)pyrimidone photolyase)
RN  - EC 4.1.99.3 (Deoxyribodipyrimidine Photo-Lyase)
SB  - IM
MH  - Chromosome Mapping
MH  - Chromosomes, Human, Pair 12
MH  - Cloning, Molecular
MH  - DNA/isolation & purification
MH  - DNA, Complementary/isolation & purification
MH  - DNA-Binding Proteins/biosynthesis/metabolism
MH  - Deoxyribodipyrimidine Photo-Lyase/*chemistry/genetics/isolation & purification
MH  - Escherichia coli/enzymology/genetics
MH  - Humans
MH  - Organ Specificity/genetics
MH  - Photoreceptor Cells/chemistry
MH  - Recombinant Fusion Proteins/isolation & purification
MH  - *Sequence Homology, Amino Acid
EDAT- 1997/10/23 00:00
MHDA- 1997/10/23 00:01
CRDT- 1997/10/23 00:00
PHST- 1997/10/23 00:00 [pubmed]
PHST- 1997/10/23 00:01 [medline]
PHST- 1997/10/23 00:00 [entrez]
AID - S0921-8777(97)00032-3 [pii]
AID - 10.1016/s0921-8777(97)00032-3 [doi]
PST - ppublish
SO  - Mutat Res. 1997 Sep;384(3):195-204. doi: 10.1016/s0921-8777(97)00032-3.