PMID- 9331966
OWN - NLM
STAT- MEDLINE
DCOM- 19980206
LR  - 20061115
IS  - 0006-2979 (Print)
IS  - 0006-2979 (Linking)
VI  - 62
IP  - 7
DP  - 1997 Jul
TI  - ADE6 gene of Saccharomyces cerevisiae yeast encoding 
      formylglycinamidine-ribonucleotide synthetase. Cloning, sequencing, and analysis.
PG  - 742-52
AB  - The ADE6 gene of Saccharomyces cerevisiae yeast encoding the enzyme 
      formylglycinamidine-ribonucleotide (FGAM)-synthetase of de novo synthesis of 
      purine nucleotides was cloned and sequenced. The gene encodes a protein 
      consisting of 1358 amino acids. The flanking regions of 1208 (5') and 728 bp (3') 
      were also sequenced. The nucleotide motif TGACTC inherent to the promotor regions 
      of other purine genes of yeast was located (-276 bp) in the 5'-region of the 
      gene. The amino acid sequence of the yeast FGAM-synthetase was found to contain 
      repeats (Leu430-Ala620 and Pro810-Ile1000). Repeats of similar patterns of 
      conserved amino acids were also detected in the structure of all other 
      FGAM-synthetases. A homology of FGAM-synthetases with certain proteins of viruses 
      from the Herpesviridae family was found.
FAU - Andreichuk YuV
AU  - Andreichuk YuV
AD  - Biological Institute, St. Petersburg State University, Russia. 
      vddomkin@vddomkin.spb.ru
FAU - Domkin, V D
AU  - Domkin VD
FAU - Ryzhova, T A
AU  - Ryzhova TA
FAU - Koulikov, V N
AU  - Koulikov VN
FAU - Kostikova, T R
AU  - Kostikova TR
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochemistry (Mosc)
JT  - Biochemistry. Biokhimiia
JID - 0376536
RN  - 0 (Viral Proteins)
RN  - EC 6.3.5.- (Carbon-Nitrogen Ligases with Glutamine as Amide-N-Donor)
RN  - EC 6.3.5.3 (phosphoribosylformylglycinamidine synthetase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Binding Sites/genetics
MH  - Carbon-Nitrogen Ligases with Glutamine as Amide-N-Donor/*genetics
MH  - Cloning, Molecular
MH  - *Genes, Fungal
MH  - Herpesviridae/genetics
MH  - Molecular Sequence Data
MH  - Restriction Mapping
MH  - Saccharomyces cerevisiae/*enzymology/*genetics
MH  - Sequence Homology, Amino Acid
MH  - Viral Proteins/genetics
EDAT- 1997/07/01 00:00
MHDA- 1997/10/23 00:01
CRDT- 1997/07/01 00:00
PHST- 1997/07/01 00:00 [pubmed]
PHST- 1997/10/23 00:01 [medline]
PHST- 1997/07/01 00:00 [entrez]
PST - ppublish
SO  - Biochemistry (Mosc). 1997 Jul;62(7):742-52.