PMID- 9331978
OWN - NLM
STAT- MEDLINE
DCOM- 19971201
LR  - 20190719
IS  - 0918-6158 (Print)
IS  - 0918-6158 (Linking)
VI  - 20
IP  - 9
DP  - 1997 Sep
TI  - Hair analysis for drugs of abuse. XVIII. 3,4-Methylenedioxymethamphetamine (MDMA) 
      disposition in hair roots and use in identification of acute MDMA poisoning.
PG  - 969-72
AB  - Disposition of 3,4-methylenedioxymethamphetamine (MDMA) in hair roots was studied 
      using rats and the hair root samples were evaluated to prove acute MDMA 
      poisoning. The back hair of male pigmented hairy rats (n = 6) was shaved and 5 d 
      later the animals were intraperitoneally administered with acute poisonous doses 
      (20, 40, 60, 80 and 100 mg/kg) of MDMA. Roots of the hairs were then plucked out 
      with a hair nipper 5 min and, 0.5, 1, 2, 6 and 24 h after injection. The hair 
      root samples were, directly or after being washed with detergent, extracted with 
      methanol-5 N HCl (20:1) under ultrasonication in ice-cold water for 4 h. After 
      filtration and evaporation, the residue was derivatized with pentafluoropropionic 
      anhydride and analyzed by GC/MS. From all samples including the 5 min sample, 
      MDMA was detected at high concentrations (up to 156 ng/mg) accompanied by 
      3,4-methylenedioxyamphetamine (MDA). Some of the animals died within 2 h after 
      administration, but in the surviving rats the MDMA concentrations in the hair 
      roots increased up to 6 h and then slowly decreased until 24 h. The remaining 
      MDMA after washing apparently increased from 13-31% at 0.5 h to 51-83% at 24 h in 
      the surviving rats. These facts show that most of drugs in the hair roots are not 
      yet immobilized in the early stage and are thereafter gradually incorporated into 
      the hair shaft. Increase of the MDMA concentration stopped soon after death of 
      the animal, probably due to the cessation of hair growth. Although the ratios of 
      MDA/MDMA steadily increased over time, those after death plateaued, probably due 
      to the cessation of metabolism after death. It was clearly shown that MDMA is 
      more quickly incorporated into and more firmly retained in hair than 
      methamphetamine (MA) by comparing their disposition in hair roots.
FAU - Nakahara, Y
AU  - Nakahara Y
AD  - National Institute of Health Sciences, Tokyo, Japan.
FAU - Kikura, R
AU  - Kikura R
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (Hallucinogens)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - Acute Disease
MH  - Animals
MH  - Disease Models, Animal
MH  - Dose-Response Relationship, Drug
MH  - Hair/*chemistry
MH  - Hallucinogens/*poisoning
MH  - Male
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*poisoning
MH  - Rats
MH  - Substance-Related Disorders/*diagnosis
EDAT- 1997/10/23 00:00
MHDA- 1997/10/23 00:01
CRDT- 1997/10/23 00:00
PHST- 1997/10/23 00:00 [pubmed]
PHST- 1997/10/23 00:01 [medline]
PHST- 1997/10/23 00:00 [entrez]
AID - 10.1248/bpb.20.969 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 1997 Sep;20(9):969-72. doi: 10.1248/bpb.20.969.