PMID- 9332304
OWN - NLM
STAT- MEDLINE
DCOM- 19971119
LR  - 20211203
IS  - 0007-1048 (Print)
IS  - 0007-1048 (Linking)
VI  - 98
IP  - 3
DP  - 1997 Sep
TI  - Granulocyte-macrophage colony-stimulating factor abrogates transforming growth 
      factor-beta 1-mediated cell cycle arrest by up-regulating cyclin D2/Cdk6.
PG  - 520-7
AB  - The role of positive and negative cytokine interactions in G1 cell cycle 
      regulation of haemopoietic cells was analysed by determination of the expression 
      patterns of D-type cyclins and cyclin-dependent kinases (cdks) in SKM-1 
      myelodysplastic syndrome (MDS) cells incubated with granulocyte-macrophage 
      colony-stimulating factor (GM-CSF) and/or transforming growth factor-beta 1 
      (TGF-beta 1). TGF-beta 1 inhibited SKM-1 cell proliferation due to the cell cycle 
      arrest in G1 phase. GM-CSF abrogated the TGF-beta 1-mediated G1 arrest in these 
      cells. Reverse transcription-polymerase chain reaction (RT-PCR) analysis 
      indicated that TGF-beta 1-mediated G1 arrest correlated with the down-regulation 
      of cdk4, cdk6 and cyclin D2, and that abrogation of TGF-beta 1-mediated G1 arrest 
      by GM-CSF correlated with the constitutive over-expression of cyclin D2 and cdk6 
      but not cdk4. These results suggest the importance of cyclin D2/cdk6 levels in 
      abrogating G1 arrest in cells exposed to TGF-beta 1, and raise the possibility 
      that the GM-CSF-mediated up-regulatory pathway of signal transduction through 
      cyclin D2/cdk6 differs from the TGF-beta 1-cdk4-mediated pathway in SKM-1 cells. 
      This signal transduction pathway through cyclin D2/cdk6 might play an important 
      role in haemopoietic regulation by the cytokine network.
FAU - Ohtsuki, F
AU  - Ohtsuki F
AD  - Department of Paediatrics, Hyogo College of Medicine, Japan.
FAU - Yamamoto, M
AU  - Yamamoto M
FAU - Nakagawa, T
AU  - Nakagawa T
FAU - Tanizawa, T
AU  - Tanizawa T
FAU - Wada, H
AU  - Wada H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Haematol
JT  - British journal of haematology
JID - 0372544
RN  - 0 (CCND2 protein, human)
RN  - 0 (CCND3 protein, human)
RN  - 0 (Cyclin D2)
RN  - 0 (Cyclin D3)
RN  - 0 (Cyclins)
RN  - 0 (Transforming Growth Factor beta)
RN  - 136601-57-5 (Cyclin D1)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.22 (CDK6 protein, human)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinase 6)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinases)
SB  - IM
MH  - Cell Cycle/drug effects
MH  - Cell Division/drug effects
MH  - Cyclin D1/metabolism
MH  - Cyclin D2
MH  - Cyclin D3
MH  - Cyclin-Dependent Kinase 6
MH  - *Cyclin-Dependent Kinases
MH  - Cyclins/metabolism
MH  - Drug Interactions
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/*pharmacology
MH  - Humans
MH  - Myelodysplastic Syndromes/pathology
MH  - Protein Serine-Threonine Kinases/*metabolism
MH  - Transforming Growth Factor beta/*pharmacology
MH  - Tumor Cells, Cultured
MH  - Up-Regulation
EDAT- 1997/10/23 00:00
MHDA- 1997/10/23 00:01
CRDT- 1997/10/23 00:00
PHST- 1997/10/23 00:00 [pubmed]
PHST- 1997/10/23 00:01 [medline]
PHST- 1997/10/23 00:00 [entrez]
AID - 10.1046/j.1365-2141.1997.2643079.x [doi]
PST - ppublish
SO  - Br J Haematol. 1997 Sep;98(3):520-7. doi: 10.1046/j.1365-2141.1997.2643079.x.