PMID- 9333393
OWN - NLM
STAT- MEDLINE
DCOM- 19971015
LR  - 20191024
IS  - 0941-293X (Print)
IS  - 0941-293X (Linking)
VI  - 94
IP  - 7
DP  - 1997 Jul
TI  - [Imaging of laser thermokeratoplasty lesions by optical low coherence tomography 
      and polarization microscopy after Sirius Red staining].
PG  - 487-91
AB  - BACKGROUND: Information on the extent and degree of the thermal effect produced 
      is of great importance for control of the laser dosage in laser 
      thermokeratoplasty (LTK) and for postoperative follow-up. We investigated on 
      acute LTK effects which information images obtained by optical low coherence 
      tomography (OCT) offer compared to those obtained by polarization microscopy. 
      METHODS: Porcine eyes were irradiated through a 400 microns quartz fiber using 
      light from a laser diode emitting up to 300 mW at a wavelength of 1.86 microns. 
      Thermal lesions of varying strength were scanned using an experimental OCT device 
      with about 25 microns lateral and 20 microns axial resolution. Histologic 
      evaluation of the scanned areas was done by polarization microscopy after 
      Sirlus-Red staining, and similar lesions were also analyzed by TEM. RESULTS: Both 
      methods differentiated three damage zones a transition zone, a zone of moderate 
      coagulation, and a central zone of strong coagulation. In the transition zone, 
      increased birefringence was seen in polarization microscopy, which correlated 
      with increased light scattering seen in the OCT images. In the moderately 
      coagulated zone, a decrease in birefringence was associated with an even stronger 
      increase of the OCT signal. In the central zone, a loss of the fibrillar tissue 
      structure was observed, which led to a complete loss of birefringence and a 
      strong reduction of the OCT signal. CONCLUSIONS: Although OCT does not provide 
      the detailed information on thermal changes of tissue seen by the histologic 
      method, it offers information on the extent and degree of tissue changes without 
      preparation artifacts and provides a non-invasive method of immediate and 
      follow-up control of LTK lesions. A quantitative analysis of changes in corneal 
      thickness and curvature is much simpler than by a slit lamp. Time-resolved 
      measurements of corneal light scattering may be used for on-line control of the 
      laser-light dosage during LTK.
FAU - Asiyo-Vogel, M N
AU  - Asiyo-Vogel MN
AD  - Augenklinik, Medizinische Universitat Lubeck.
FAU - Koop, N
AU  - Koop N
FAU - Brinkmann, R
AU  - Brinkmann R
FAU - Engelhardt, R
AU  - Engelhardt R
FAU - Eggers, R
AU  - Eggers R
FAU - Birngruber, R
AU  - Birngruber R
FAU - Vogel, A
AU  - Vogel A
LA  - ger
PT  - English Abstract
PT  - Journal Article
TT  - Darstellung von LTK-Lasionen durch optische Kurzkoharenztomographie (OCT) und 
      Polarisationsmikroskopie nach Sirius-Rot-Farbung.
PL  - Germany
TA  - Ophthalmologe
JT  - Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft
JID - 9206148
RN  - 0 (Azo Compounds)
RN  - 0 (Coloring Agents)
RN  - 1294D5G72N (C.I. direct red 80)
SB  - IM
MH  - Animals
MH  - *Azo Compounds
MH  - *Coloring Agents
MH  - Cornea/pathology/*surgery
MH  - Corneal Stroma/pathology/surgery
MH  - Lasers, Excimer
MH  - Microscopy, Electron
MH  - Microscopy, Polarization/*instrumentation
MH  - Photorefractive Keratectomy/*instrumentation
MH  - Swine
MH  - Tomography/*instrumentation
EDAT- 1997/07/01 00:00
MHDA- 1997/10/23 00:01
CRDT- 1997/07/01 00:00
PHST- 1997/07/01 00:00 [pubmed]
PHST- 1997/10/23 00:01 [medline]
PHST- 1997/07/01 00:00 [entrez]
AID - 10.1007/s003470050144 [doi]
PST - ppublish
SO  - Ophthalmologe. 1997 Jul;94(7):487-91. doi: 10.1007/s003470050144.