PMID- 9334402
OWN - NLM
STAT- MEDLINE
DCOM- 19971120
LR  - 20231012
IS  - 0270-6474 (Print)
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Linking)
VI  - 17
IP  - 21
DP  - 1997 Nov 1
TI  - Analysis of rat vestibular hair cell development and regeneration using 
      calretinin as an early marker.
PG  - 8270-82
AB  - Despite increased interest in inner ear hair cell regeneration, it is still 
      unclear what exact mechanisms underlie hair cell regeneration in mammals because 
      of our limited understanding of hair cell development and the lack of specific 
      hair cell markers. In this report, we studied hair cell development using 
      immunohistochemistry on sections prepared from embryonic day (E) 13 to postnatal 
      day 7 rat inner ear tissues. Of many epithelial, neuronal, and glial markers, we 
      found that calcium-binding protein antibodies recognizing calretinin, calmodulin, 
      or parvalbumin labeled immature hair cells in rat vestibular end organs. In 
      particular, calretinin antiserum labeled the initial differentiating hair cells 
      at E15, a stage immediately after the terminal mitosis of hair cell progenitors. 
      The selective immunoreactivity of postmitotic presumptive hair cells, but not 
      supporting cells or peripheral epithelial cells, was confirmed in utricular 
      epithelial sheet cultures. Double labeling with calretinin and bromodeoxyuridine 
      antibodies in long-term cultures showed that only a few mitotic utricular 
      supporting cells became calretinin positive. Thus, although 
      proliferation-mediated regeneration of new hair cells might directly contribute 
      to hair cell regeneration in rat utricles after injury, it is very limited. In 
      addition, double labeling with calretinin and terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) revealed that differentiated 
      hair cells underwent apoptosis during normal development at late embryonic and 
      early postnatal stages in vivo and in vitro. Therefore, these experiments lay the 
      groundwork for the time course of differentiation, regeneration, and apoptosis of 
      mammalian vestibular hair cells. This work also suggests that calcium-binding 
      proteins are useful markers for studies on inner ear hair cell differentiation 
      and regeneration.
FAU - Zheng, J L
AU  - Zheng JL
AD  - Department of Neuroscience, Genentech, Inc., South San Francisco, California 
      94080, USA.
FAU - Gao, W Q
AU  - Gao WQ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Biomarkers)
RN  - 0 (Calb2 protein, rat)
RN  - 0 (Calbindin 2)
RN  - 0 (Calmodulin)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Parvalbumins)
RN  - 0 (S100 Calcium Binding Protein G)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Biomarkers
MH  - Bromodeoxyuridine/analysis
MH  - Calbindin 2
MH  - Calmodulin/analysis
MH  - Cell Differentiation
MH  - Cell Division
MH  - Cells, Cultured
MH  - Gene Expression Regulation, Developmental
MH  - Hair Cells, Vestibular/chemistry/*cytology
MH  - Nerve Regeneration
MH  - Nerve Tissue Proteins/*analysis
MH  - Parvalbumins/analysis
MH  - Rats
MH  - Rats, Wistar
MH  - S100 Calcium Binding Protein G/*analysis/genetics
MH  - Saccule and Utricle/cytology
MH  - Vestibule, Labyrinth/cytology/embryology/*growth & development/physiology
PMC - PMC6573764
EDAT- 1997/10/23 00:00
MHDA- 1997/10/23 00:01
PMCR- 1998/05/01
CRDT- 1997/10/23 00:00
PHST- 1997/10/23 00:00 [pubmed]
PHST- 1997/10/23 00:01 [medline]
PHST- 1997/10/23 00:00 [entrez]
PHST- 1998/05/01 00:00 [pmc-release]
AID - 10.1523/JNEUROSCI.17-21-08270.1997 [doi]
PST - ppublish
SO  - J Neurosci. 1997 Nov 1;17(21):8270-82. doi: 10.1523/JNEUROSCI.17-21-08270.1997.