PMID- 9335004
OWN - NLM
STAT- MEDLINE
DCOM- 19971120
LR  - 20190724
IS  - 0022-510X (Print)
IS  - 0022-510X (Linking)
VI  - 151
IP  - 1
DP  - 1997 Oct 3
TI  - Schwann cell extracellular matrix protein production is modulated by 
      Mycobacterium leprae and macrophage secretory products.
PG  - 13-22
AB  - Extracellular matrix (ECM) protein deposition is an important feature of leprous 
      nerves, where Schwann cells (SCs) and macrophages are the main hosts for 
      Mycobacterium leprae. Since, SCs are involved in the synthesis of ECM proteins 
      and its production is regulated by macrophage secretory factors, the present 
      study aimed to determine in vitro, the effect of M. leprae infection and 
      macrophage secretory products on secretion of ECM proteins by SCs in two strains 
      of mice, Swiss White (SW) and C57BL/6, that are known to differ in their nerve 
      pathology and macrophage functions in response to infection. Following six days 
      of M. leprae infection, SCs from SW mice responded with increased secretion of 
      14C-leucine radiolabelled proteins and a concomitant increase in laminin and 
      collagens type I, III and IV, as determined by enzyme-linked immunosorbent assay. 
      In contrast infected C57BL/6 SCs responded with decreased secretion of total 
      proteins and fibronectin. Exposure of SCs to macrophage conditioned medium 
      resulted in decreased ECM protein secretion in both strains of mice. This 
      decrease was a function of protein breakdown by macrophage derived proteases and 
      also active regulation by macrophage secreted cytokines. A similar effect of M. 
      leprae and macrophage secretory products on SC metabolism in leprous nerves would 
      have major ramifications on damage and repair activities. In addition ECM 
      proteins would also influence the composition of the infiltrating cell population 
      in lepromatous and tuberculoid nerves.
FAU - Singh, N
AU  - Singh N
AD  - Foundation for Medical Research, Bombay, India.
FAU - Birdi, T J
AU  - Birdi TJ
FAU - Chandrashekar, S
AU  - Chandrashekar S
FAU - Antia, N H
AU  - Antia NH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Neurol Sci
JT  - Journal of the neurological sciences
JID - 0375403
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Fibronectins)
RN  - 0 (Laminin)
RN  - 0 (Membrane Glycoproteins)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Collagen/metabolism
MH  - Extracellular Matrix Proteins/*biosynthesis
MH  - Fibronectins/metabolism
MH  - Laminin/analysis
MH  - Leprosy/*metabolism
MH  - Macrophages, Peritoneal/*metabolism
MH  - Membrane Glycoproteins/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mycobacterium leprae/*metabolism
MH  - Schwann Cells/*metabolism
EDAT- 1997/10/23 00:00
MHDA- 1997/10/23 00:01
CRDT- 1997/10/23 00:00
PHST- 1997/10/23 00:00 [pubmed]
PHST- 1997/10/23 00:01 [medline]
PHST- 1997/10/23 00:00 [entrez]
AID - S0022-510X(97)00105-6 [pii]
AID - 10.1016/s0022-510x(97)00105-6 [doi]
PST - ppublish
SO  - J Neurol Sci. 1997 Oct 3;151(1):13-22. doi: 10.1016/s0022-510x(97)00105-6.