PMID- 9335367
OWN - NLM
STAT- MEDLINE
DCOM- 19980202
LR  - 20190831
IS  - 0160-564X (Print)
IS  - 0160-564X (Linking)
VI  - 21
IP  - 10
DP  - 1997 Oct
TI  - Phenotypic alterations in circulating monocytes induced by open heart surgery 
      using heparinized and nonheparinized cardiopulmonary bypass systems.
PG  - 1091-7
AB  - In this study of 31 patients with coronary bypass surgery, we used flow cytometry 
      to compare heparin-coated and noncoated cardiopulmonary bypass systems on 
      leukocyte activation. We found significant differences between the groups during 
      bypass, with activation of the complement system, measured as elevated levels of 
      C3a desArg, upregulation of granulocyte beta2 integrin (CD11b), and a loss of 
      circulating monocytes when noncoated systems were used. In both groups an early 
      increase in the monocyte cell surface CD62L expression was obvious while the 
      percentage of human leukocyte antigen (HLA)-DR positive monocytes did not alter. 
      The morning after the operation, leukocytosis was present, together with a highly 
      significant reduction in the monocyte expression of CD11b and HLA-DR, indicating 
      the recruitment to the peripheral blood of cells with altered phenotypes. This 
      alteration in phenotype on potent inflammatory cells may be one part of the 
      impaired function of the immunological system reported after major surgery.
FAU - Ljunghusen, O
AU  - Ljunghusen O
AD  - Department of Medical Microbiology and Immunology, University Hospital, 
      Linkoping, Sweden.
FAU - Cederholm, I
AU  - Cederholm I
FAU - Lundahl, J
AU  - Lundahl J
FAU - Nilsson, B
AU  - Nilsson B
FAU - Olin, C
AU  - Olin C
FAU - Sjogren, F
AU  - Sjogren F
FAU - Stendahl, O
AU  - Stendahl O
LA  - eng
PT  - Clinical Trial
PT  - Comparative Study
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Artif Organs
JT  - Artificial organs
JID - 7802778
RN  - 0 (Anaphylatoxins)
RN  - 0 (Fibrinolytic Agents)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Macrophage-1 Antigen)
RN  - 0 (complement C3a, des-Arg-(77)-)
RN  - 126880-86-2 (L-Selectin)
RN  - 80295-42-7 (Complement C3a)
RN  - 9005-49-6 (Heparin)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Anaphylatoxins/analysis
MH  - Cardiopulmonary Bypass/*standards
MH  - Complement Activation/drug effects/genetics
MH  - Complement C3a/analogs & derivatives/analysis
MH  - Female
MH  - Fibrinolytic Agents/administration & dosage/*pharmacology
MH  - Flow Cytometry
MH  - Gene Expression Regulation/drug effects/genetics
MH  - Granulocytes/drug effects/immunology
MH  - HLA-DR Antigens/*blood
MH  - Heparin/administration & dosage/*pharmacology
MH  - Humans
MH  - L-Selectin/blood/genetics
MH  - Lymphocyte Activation/*drug effects
MH  - Macrophage-1 Antigen/blood
MH  - Male
MH  - Middle Aged
MH  - Monocytes/*drug effects/immunology
MH  - Phenotype
MH  - Up-Regulation
EDAT- 1997/10/23 00:00
MHDA- 1997/10/23 00:01
CRDT- 1997/10/23 00:00
PHST- 1997/10/23 00:00 [pubmed]
PHST- 1997/10/23 00:01 [medline]
PHST- 1997/10/23 00:00 [entrez]
AID - 10.1111/j.1525-1594.1997.tb00448.x [doi]
PST - ppublish
SO  - Artif Organs. 1997 Oct;21(10):1091-7. doi: 10.1111/j.1525-1594.1997.tb00448.x.