PMID- 9338437
OWN - NLM
STAT- MEDLINE
DCOM- 19971126
LR  - 20171213
IS  - 8750-7587 (Print)
IS  - 0161-7567 (Linking)
VI  - 83
IP  - 4
DP  - 1997 Oct
TI  - Passive sensitization of human airways induces myogenic contractile responses in 
      vitro.
PG  - 1276-81
AB  - We assessed effects of passive sensitization on human bronchial smooth muscle 
      (BSM) response to mechanical stretching in vitro. Bronchial rings were sham 
      (control) or passively sensitized overnight by using sera from donors 
      demonstrating sensitivity to Dermatophagoides farinae and having immunoglobulin E 
      (IgE) concentrations of 2,600 +/- 200 U/ml. Tissues were fixed isometrically to 
      force transducers to measure responses to electrical field stimulation (EFS) and 
      quick stretch (QS). The myogenic response to QS was normalized to the maximal 
      response to EFS (%EFS). The myogenic response of sensitized BSM was 47.9 +/- 10.9 
      %EFS to a QS of approximately 6.5% optimal length (Lo); sham-sensitized tissues 
      had a myogenic response of 13.5 +/- 6.4 %EFS (P = 0.012 vs. passively 
      sensitized). A QS of approximately 13% Lo in sensitized BSM caused a response of 
      82.8 +/- 20.9 %EFS; sham-sensitized tissues developed a response of 38.2 +/- 17.3 
      %EFS (P = 0.004). BSM incubated with serum from nonallergic donors did not 
      demonstrate increased QS response (4.6 +/- 1.4 %EFS, P = not significant vs. 
      tissue exposed to atopic sera). However, tissues incubated in sera from nonatopic 
      donors supplemented with hapten-specific chimeric IgE (JW8) demonstrated 
      augmented myogenic response to QS of approximately 6.5% Lo (21.9 +/- 6.2 %EFS, P 
      = 0. 027 vs. nonatopic sera alone). We demonstrate that passive sensitization of 
      human BSM preparations causes induction and augmentation of myogenic contractions 
      to QS; this hyperresponsiveness corresponds to the IgE concentration in 
      sensitizing sera.
FAU - Mitchell, R W
AU  - Mitchell RW
AD  - Asthma, Allergy, and Immunological Disease Research Center, The University of 
      Chicago, Chicago, Illinois 60637, USA.
FAU - Rabe, K F
AU  - Rabe KF
FAU - Magnussen, H
AU  - Magnussen H
FAU - Leff, A R
AU  - Leff AR
LA  - eng
GR  - HL-46368/HL/NHLBI NIH HHS/United States
GR  - HL-56399/HL/NHLBI NIH HHS/United States
GR  - NAIAD-AI-34566/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Appl Physiol (1985)
JT  - Journal of applied physiology (Bethesda, Md. : 1985)
JID - 8502536
RN  - 0 (Allergens)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Allergens/toxicity
MH  - Bronchi/immunology/*physiopathology
MH  - Electric Stimulation
MH  - Humans
MH  - *Immunization, Passive
MH  - Immunoglobulin E/immunology/physiology
MH  - In Vitro Techniques
MH  - Muscle Contraction/physiology
MH  - Muscle, Smooth/*physiopathology
MH  - Pulmonary Stretch Receptors/physiology
MH  - Respiratory Hypersensitivity/immunology/*physiopathology
EDAT- 1997/10/24 00:00
MHDA- 1997/10/24 00:01
CRDT- 1997/10/24 00:00
PHST- 1997/10/24 00:00 [pubmed]
PHST- 1997/10/24 00:01 [medline]
PHST- 1997/10/24 00:00 [entrez]
AID - 10.1152/jappl.1997.83.4.1276 [doi]
PST - ppublish
SO  - J Appl Physiol (1985). 1997 Oct;83(4):1276-81. doi: 10.1152/jappl.1997.83.4.1276.