PMID- 9339848
OWN - NLM
STAT- MEDLINE
DCOM- 19971208
LR  - 20061115
IS  - 0889-2229 (Print)
IS  - 0889-2229 (Linking)
VI  - 13
IP  - 15
DP  - 1997 Oct 10
TI  - HIV type 1 envelope glycoprotein 120 carboxy-terminal peptide-induced human T 
      cell lines selectively suppress heterogeneous proliferative T cell responses to 
      soluble antigens.
PG  - 1313-24
AB  - It has been proposed that the highly conserved human immunodeficiency virus type 
      1 (HIV-1) envelope gp120 carboxy-terminal sequence, TKAKRRVVEREKR (CT120), may 
      represent a functional mimic of the human leukocyte antigen (HLA) class II DR 
      beta-chain third hypervariable region (HVR3) sequence motif located at position 
      69-81. Presentation of this potentially pathogenic fragment by HLA class I and/or 
      II molecules, in a manner analogous to the indirect pathway of allorecognition, 
      may induce both widespread cellular activation and also break self-tolerance, 
      resulting in the selective and progressive anti-self HLA class II-directed immune 
      suppression, which is a central feature of HIV-1 infection and the associated 
      acquired immune deficiency syndrome (AIDS). To investigate the functional role of 
      the HIV-1 gp120 C-terminal fragment T cell lines (TCLs) were raised from three 
      healthy HIV-1-seronegative subjects at low risk of HIV-1 exposure, by repeated 
      stimulation with a short synthetic 13-mer CT120 peptide in vitro. Graded 
      concentrations (10[3] to 5 x 10[4]) of CT120 TCLs suppressed the primary 6-day 
      proliferation of autologous PBMCs in response to the soluble antigens tetanus 
      toxoid (TT) and purified protein derivative (PPD). In contrast, CT120 TCLs 
      demonstrated no suppressive effect on 3-day phytohemagglutinin (PHA), 
      concanavalin A (ConA), and pokeweed mitogen (PWM) mitogenic responses. 
      Fractionation of CT120 TCLs into highly purified CD4+ and CD8+ T cell subsets 
      demonstrated that the CD8+ T cell fraction mediated the suppressor effector 
      function. HLA restriction analysis revealed a complex pattern as both anti-HLA 
      class II DR and anti-HLA class I (A, B, C) MAbs inhibited proliferation of 
      oligoclonal CD8+ CT120 TCLs. Strategies aimed at specifically inhibiting such 
      putative immunopathogenic HIV-1-encoded T cell epitopes may be an important 
      consideration for development of future HIV-1 immunotherapy.
FAU - Wilson, S E
AU  - Wilson SE
AD  - Academic Virology, The London Hospital Medical College, UK. 
      swilson@hivnet.fhcrc.org
FAU - Habeshaw, J A
AU  - Habeshaw JA
FAU - Addawe, M A
AU  - Addawe MA
FAU - Hounsell, E F
AU  - Hounsell EF
FAU - Oxford, J S
AU  - Oxford JS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - AIDS Res Hum Retroviruses
JT  - AIDS research and human retroviruses
JID - 8709376
RN  - 0 (Antibodies, Blocking)
RN  - 0 (Epitopes)
RN  - 0 (HIV Envelope Protein gp120)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Peptides)
RN  - 0 (Phytohemagglutinins)
RN  - 0 (Pokeweed Mitogens)
RN  - 0 (Tetanus Toxoid)
RN  - 0 (Tuberculin)
RN  - 11028-71-0 (Concanavalin A)
SB  - IM
MH  - Adult
MH  - Amino Acid Sequence
MH  - Antibodies, Blocking/immunology
MH  - Autoimmunity
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Cell Division
MH  - Cells, Cultured
MH  - Concanavalin A/immunology
MH  - Cross Reactions/immunology
MH  - Dose-Response Relationship, Immunologic
MH  - Epitope Mapping
MH  - Epitopes/immunology
MH  - HIV Envelope Protein gp120/*immunology
MH  - HIV Infections/*immunology
MH  - HIV Seronegativity
MH  - HIV-1/*immunology
MH  - Histocompatibility Antigens Class I/immunology
MH  - Histocompatibility Antigens Class II/immunology
MH  - Histocompatibility Testing
MH  - Humans
MH  - Immune Tolerance
MH  - Leukocytes, Mononuclear/immunology
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Peptides/chemical synthesis/*immunology
MH  - Phytohemagglutinins/immunology
MH  - Pokeweed Mitogens/immunology
MH  - T-Lymphocyte Subsets/immunology
MH  - T-Lymphocytes/cytology/*immunology
MH  - Tetanus Toxoid/immunology
MH  - Tuberculin/immunology
EDAT- 1997/10/27 00:00
MHDA- 1997/10/27 00:01
CRDT- 1997/10/27 00:00
PHST- 1997/10/27 00:00 [pubmed]
PHST- 1997/10/27 00:01 [medline]
PHST- 1997/10/27 00:00 [entrez]
AID - 10.1089/aid.1997.13.1313 [doi]
PST - ppublish
SO  - AIDS Res Hum Retroviruses. 1997 Oct 10;13(15):1313-24. doi: 
      10.1089/aid.1997.13.1313.