PMID- 9342622
OWN - NLM
STAT- MEDLINE
DCOM- 19971205
LR  - 20220310
IS  - 0340-0131 (Print)
IS  - 0340-0131 (Linking)
VI  - 70
IP  - 4
DP  - 1997
TI  - Biological monitoring of workers exposed to low levels of 2-butoxyethanol.
PG  - 232-6
AB  - OBJECTIVES: (1) To assess the value of urinary butoxyacetic acid (BAA) 
      measurement for the monitoring of workers exposed to low concentration of 
      2-butoxyethanol (BE); (2) to evaluate the in vivo effect of low occupational BE 
      exposure on the erythrocyte lineage; and (3) to test the possible influence of 
      genetic polymorphism for cytochrome P450 2E1 (CYP 2E1) on urinary BAA excretion 
      rate. METHODS: Thirty-one male workers exposed to BE in a beverage package 
      production plant were examined according to their external (BE) and internal 
      (BAA) solvent exposure. The effect of this exposure on erythrocyte lineage [red 
      blood cell numeration (RBC), hemoglobin (Hb), hematocrit (Htc), mean corpuscular 
      volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin 
      concentration (MCHC), haptoglobin (Hp), reticulocyte numeration (Ret) and osmotic 
      resistance (OR)], hepatic [aspartate aminotransferase (GOT), alanine 
      aminotransferase (GPT)] and renal [plasmatic creatinine, urinary retinol binding 
      protein (RBP)] parameters was also investigated. DNA purified from whole blood 
      was used for CYP 2E1 genotyping. RESULTS: Average airborne concentration of BE 
      was 2.91 mg/m3 (0.59 ppm) with a standard deviation of 1.30 mg/m3 (0.27 ppm). 
      There was a relatively good correlation between external and internal exposure 
      estimated by measuring BAA in post-shift urine samples (average 10.4 mg/g 
      creatinine; r = 0.55; P = 0.0012). Compared with a matched control group (n = 21) 
      exposed workers had a statistically significant decrease (3.3%; P = 0.03) in Hct 
      while MCHC was increased (2.1%; P = 0.02). No significant difference was observed 
      either in other erythroid parameters or in hepatic and renal biomarkers. One 
      exposed individual exhibited a mutant allele with increased cytochrome P450 
      oxidative activity which coincided with a very low urinary BAA excretion. 
      CONCLUSIONS: Single determination of BAA in post-shift urine samples can be used 
      to assess exposure to low levels of BE. A slight but significant effect on 
      erythroid parameters suggesting membrane damage was observed in exposed workers. 
      The influence of the genetic polymorphism for CYP 2E1 deserves further 
      investigation for the interpretation of urinary BAA measurements.
FAU - Haufroid, V
AU  - Haufroid V
AD  - Universite Catholique de Louvain, Faculte de Medecine, Ecole de Sante Publique, 
      Unite de Toxicologie Industrielle et de Medecine du Travail, Bruxelles, Belgium.
FAU - Thirion, F
AU  - Thirion F
FAU - Mertens, P
AU  - Mertens P
FAU - Buchet, J P
AU  - Buchet JP
FAU - Lison, D
AU  - Lison D
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Int Arch Occup Environ Health
JT  - International archives of occupational and environmental health
JID - 7512134
RN  - 0 (Air Pollutants, Occupational)
RN  - 0 (Ethylene Glycols)
RN  - 0 (Solvents)
RN  - EC 1.14.13.- (Cytochrome P-450 CYP2E1)
RN  - EC 2.6.1.1 (Aspartate Aminotransferases)
RN  - EC 2.6.1.2 (Alanine Transaminase)
RN  - I0P9XEZ9WV (n-butoxyethanol)
SB  - IM
MH  - Adult
MH  - Air Pollutants, Occupational/adverse effects/*pharmacokinetics
MH  - Alanine Transaminase/blood
MH  - Aspartate Aminotransferases/blood
MH  - Cytochrome P-450 CYP2E1/genetics
MH  - Dose-Response Relationship, Drug
MH  - *Environmental Monitoring
MH  - Erythrocyte Membrane/drug effects
MH  - Erythrocytes/drug effects
MH  - Ethylene Glycols/adverse effects/*pharmacokinetics
MH  - Humans
MH  - Liver Function Tests
MH  - Male
MH  - Middle Aged
MH  - Occupational Diseases/chemically induced/diagnosis/*urine
MH  - Occupational Exposure/*analysis
MH  - Polymorphism, Genetic/genetics
MH  - Solvents/adverse effects/*pharmacokinetics
EDAT- 1997/01/01 00:00
MHDA- 1997/10/29 00:01
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1997/10/29 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
AID - 10.1007/s004200050212 [doi]
PST - ppublish
SO  - Int Arch Occup Environ Health. 1997;70(4):232-6. doi: 10.1007/s004200050212.