PMID- 9342702
OWN - NLM
STAT- MEDLINE
DCOM- 19980112
LR  - 20190516
IS  - 0916-7250 (Print)
IS  - 0916-7250 (Linking)
VI  - 59
IP  - 9
DP  - 1997 Sep
TI  - Apoptosis of murine hepatocytes induced by high doses of galactosamine.
PG  - 785-90
AB  - Apoptosis induced by high doses of Galactosamine (GalN) was investigated in mice 
      hepatocytes in vivo. In mice intraperitoneally (i.p.) treated with GalN 3 g/kg, 
      the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling 
      (TUNEL)-positive cells were first observed at 6 hr postadministration (PA). Both 
      acidophilic bodies in hematoxylin and eosin (HE)-stained sections and 
      TUNEL-positive cells were markedly found at 24 hr PA. At 48 hr PA, cellular 
      degeneration and necrosis of hepatocytes were prominently observed, and 
      TUNEL-positive cells were scarcely found. In the mice ip treated with GalN 1.5 
      g/kg, the lesion was milder than that in those treated with GalN 3 g/kg. 
      Acidophilic bodies and TUNEL-positive cells were scarcely found at 24 hr PA, 
      whereas they were markedly seen at 48 hr PA. In addition, a ladder-like DNA 
      fragmentation pattern by agarose gel electrophoresis was observed most remarkably 
      at 24 hr PA with GalN 3 g/kg and at 48 hr PA with GalN 1.5 g/kg, and less 
      distinctly at 48 hr PA with GalN 3 g/kg. On the other hand, sGOT and sGPT 
      activities increased prominently at 48 hr PA with GalN 3 g/kg. These results 
      suggest that the cell death induced by high dose of GalN may be caused by 
      apoptosis, and subsequently by necrosis in vivo.
FAU - Tsutsui, S
AU  - Tsutsui S
AD  - Department of Biomedical Science, Faculty of Agriculture, University of Tokyo, 
      Japan.
FAU - Hirasawa, K
AU  - Hirasawa K
FAU - Takeda, M
AU  - Takeda M
FAU - Itagaki, S
AU  - Itagaki S
FAU - Kawamura, S
AU  - Kawamura S
FAU - Maeda, K
AU  - Maeda K
FAU - Mikami, T
AU  - Mikami T
FAU - Doi, K
AU  - Doi K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - J Vet Med Sci
JT  - The Journal of veterinary medical science
JID - 9105360
RN  - 7535-00-4 (Galactosamine)
RN  - EC 2.6.1.1 (Aspartate Aminotransferases)
RN  - EC 2.6.1.2 (Alanine Transaminase)
SB  - IM
MH  - Alanine Transaminase/blood
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Aspartate Aminotransferases/blood
MH  - Chemical and Drug Induced Liver Injury
MH  - DNA Fragmentation
MH  - Dose-Response Relationship, Drug
MH  - Electrophoresis, Agar Gel/methods/veterinary
MH  - Galactosamine/administration & dosage/*pharmacology
MH  - Histocytochemistry/methods
MH  - Injections, Intraperitoneal/veterinary
MH  - Liver/drug effects/*pathology/ultrastructure
MH  - Liver Diseases/blood/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microscopy, Electron/methods/veterinary
MH  - Necrosis
MH  - Time Factors
EDAT- 1997/10/29 00:00
MHDA- 1997/10/29 00:01
CRDT- 1997/10/29 00:00
PHST- 1997/10/29 00:00 [pubmed]
PHST- 1997/10/29 00:01 [medline]
PHST- 1997/10/29 00:00 [entrez]
AID - 10.1292/jvms.59.785 [doi]
PST - ppublish
SO  - J Vet Med Sci. 1997 Sep;59(9):785-90. doi: 10.1292/jvms.59.785.