PMID- 9343194
OWN - NLM
STAT- MEDLINE
DCOM- 19971113
LR  - 20201209
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 71
IP  - 11
DP  - 1997 Nov
TI  - Mutations in coat protein binding sites of alfalfa mosaic virus RNA 3 affect 
      subgenomic RNA 4 accumulation and encapsidation of viral RNAs.
PG  - 8385-91
AB  - The 3'-untranslated regions (3'-UTRs) of the three RNAs of alfalfa mosaic virus 
      (AMV) contain a specific binding site for coat protein (CP) and act as a promoter 
      for minus-strand RNA synthesis by the purified AMV RNA-dependent RNA polymerase 
      (RdRp) in an in vitro assay. Binding of CP to the viral RNAs is required to 
      initiate infection. The sequence of the 3'-terminal 39 nucleotides of AMV RNA 3 
      can be folded into two stem-loop structures flanked by three single-stranded AUGC 
      sequences and represents a CP binding site. Mutations in this sequence that are 
      known to interfere with CP binding in vitro were introduced into an infectious 
      clone of RNA 3, and mutant RNA transcripts were used as templates in the in vitro 
      RdRp assay and to infect protoplasts and plants. Mutation of AUGC motif 2 or 
      disruption of the stem of the 3'-proximal hairpin 1 interfered with CP binding in 
      vitro but not with minus-strand promoter activity in vitro or replication of RNA 
      3 in vivo. However, hairpin 1 appeared to be essential for encapsidation of RNA 
      3. Reversion of three G-C base pairs in hairpin 1 had no effect on CP binding but 
      interfered with minus-strand promoter activity in vitro and with RNA 3 
      replication in vivo. It is concluded that the viral RdRp and CP recognize 
      different elements in the 3'-UTRs of AMV RNAs. Moreover, several mutations that 
      interfered with CP binding in vitro interfered with the accumulation in vivo of 
      RNA 4, the subgenomic messenger for CP, but not with the accumulation of RNA 3.
FAU - Reusken, C B
AU  - Reusken CB
AD  - Institute of Molecular Plant Sciences, Gorlaeus Laboratories, Leiden University, 
      The Netherlands.
FAU - Neeleman, L
AU  - Neeleman L
FAU - Brederode, F T
AU  - Brederode FT
FAU - Bol, J F
AU  - Bol JF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (RNA, Viral)
RN  - EC 2.7.7.48 (RNA-Dependent RNA Polymerase)
SB  - IM
MH  - Alfalfa mosaic virus/genetics/*growth & development
MH  - Base Sequence
MH  - Binding Sites
MH  - Capsid/*metabolism/*ultrastructure
MH  - Gene Expression Regulation, Viral
MH  - Molecular Sequence Data
MH  - Morphogenesis
MH  - Nucleic Acid Conformation
MH  - RNA, Viral/*metabolism
MH  - RNA-Dependent RNA Polymerase/metabolism
MH  - Structure-Activity Relationship
MH  - Virion/metabolism
PMC - PMC192300
EDAT- 1997/10/29 00:00
MHDA- 1997/10/29 00:01
PMCR- 1997/11/01
CRDT- 1997/10/29 00:00
PHST- 1997/10/29 00:00 [pubmed]
PHST- 1997/10/29 00:01 [medline]
PHST- 1997/10/29 00:00 [entrez]
PHST- 1997/11/01 00:00 [pmc-release]
AID - 10.1128/JVI.71.11.8385-8391.1997 [doi]
PST - ppublish
SO  - J Virol. 1997 Nov;71(11):8385-91. doi: 10.1128/JVI.71.11.8385-8391.1997.