PMID- 9343305
OWN - NLM
STAT- MEDLINE
DCOM- 19980205
LR  - 20191024
IS  - 0952-5041 (Print)
IS  - 0952-5041 (Linking)
VI  - 19
IP  - 2
DP  - 1997 Oct
TI  - The regulation of mammary prolactin receptor metabolism by a retroviral envelope 
      protein.
PG  - 131-6
AB  - In a previous study, infection with the mouse mammary tumor virus (MMTV) was 
      shown to increase the sensitivity of the mammary epithelium toward prolactin 
      (PRL); furthermore, this effect could be mimicked by the binding of the MMTV 
      envelope protein (gp52) to its cell receptor. The present work has investigated 
      the possibility that gp52-induced changes in the PRL receptor (PRLR) were 
      responsible for this phenomenon. In vitro, gp52 doubled the PRLR concentration in 
      the plasmalemma of mammary epithelium without affecting the affinity. The origins 
      of these PRLRs were twofold: first, gp52 stimulated PRLR mRNA nearly fivefold, 
      suggesting that some of the receptors were newly synthesized. Second, there was a 
      redistribution of PRLRs within the mammary cell: PRLRs were shifted from an 
      internal pool to the plasma membrane. This relocation was very rapid, occurring 
      within 30 min. There did not appear to be any contribution from alterations in 
      PRLR degradation, since the half-life of PRLR was not affected by gp52. In 
      summary, the MMTV increases the PRL sensitivity of mouse mammary epithelium by 
      elevating PRLRs through both enhanced synthesis and recruitment from microsomes.
FAU - Bolander, F F Jr
AU  - Bolander FF Jr
AD  - Department of Biological Sciences, University of South Carolina, Columbia 29208, 
      USA.
FAU - Ginsburg, E
AU  - Ginsburg E
FAU - Vonderhaar, B K
AU  - Vonderhaar BK
LA  - eng
GR  - CA 42009/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Mol Endocrinol
JT  - Journal of molecular endocrinology
JID - 8902617
RN  - 0 (Antigens, Viral, Tumor)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Prolactin)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein 52 antigen, Mouse mammary tumor virus)
SB  - IM
MH  - Animals
MH  - Antigens, Viral, Tumor/*physiology
MH  - Cell Membrane/metabolism
MH  - Epithelial Cells/metabolism
MH  - Female
MH  - Mammary Glands, Animal/drug effects/*metabolism/virology
MH  - Mammary Tumor Virus, Mouse/*physiology
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Organ Culture Techniques
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/analysis
MH  - Receptors, Prolactin/biosynthesis/genetics/*metabolism
MH  - Viral Envelope Proteins/*physiology
EDAT- 1997/10/29 17:17
MHDA- 2001/03/28 10:01
CRDT- 1997/10/29 17:17
PHST- 1997/10/29 17:17 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1997/10/29 17:17 [entrez]
AID - 10.1677/jme.0.0190131 [doi]
PST - ppublish
SO  - J Mol Endocrinol. 1997 Oct;19(2):131-6. doi: 10.1677/jme.0.0190131.