PMID- 9344355
OWN - NLM
STAT- MEDLINE
DCOM- 19971030
LR  - 20061115
IS  - 0146-0404 (Print)
IS  - 0146-0404 (Linking)
VI  - 38
IP  - 11
DP  - 1997 Oct
TI  - Apoptotic cell death in rabbit lens after lens extraction.
PG  - 2322-30
AB  - PURPOSE: To determine whether apoptosis or necrosis of lens epithelial cells 
      occurs after lens extraction. METHODS: Lens extraction was performed on 24 rabbit 
      eyes. The authors then performed terminal deoxynucleotidyl transferase-mediated 
      dUTP nick end labeling (TUNEL) and transmission electron microscopy on the eyes 1 
      to 10 days after surgery. RESULTS: The postoperative lens was demarcated into 
      three regions by the adhesion between the incised edge of the anterior capsule 
      and the posterior capsule: the adhesive region, the intracapsular region, and the 
      central portion of the posterior capsule. By day 2, TUNEL-positive cells with 
      morphologic characteristics of necrosis were detected in the intracapsular 
      region. Beginning on day 5, TUNEL-positive cells with morphologic characteristics 
      of apoptosis and necrosis were detected in the adhesive region. Apoptotic cell 
      death in this region was detected among the myofibroblast-like cells. Lens 
      epithelial cells that had extended onto the central portion of the posterior 
      capsule began to diminish on day 8 or 9, some of them morphologically 
      demonstrating necrotic changes. CONCLUSIONS: Apoptosis of lens epithelial cells 
      occurs in the process of wound healing and reepithelialization after lens 
      extraction, leading to secondary cataract. If the induction of apoptosis is 
      better understood, protocols might be developed that could prevent 
      reepithelialization through apoptosis, thus delaying or preventing secondary 
      cataracts.
FAU - Kato, K
AU  - Kato K
AD  - Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan.
FAU - Kurosaka, D
AU  - Kurosaka D
FAU - Nagamoto, T
AU  - Nagamoto T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Invest Ophthalmol Vis Sci
JT  - Investigative ophthalmology & visual science
JID - 7703701
RN  - 0 (Deoxyuracil Nucleotides)
RN  - EC 2.7.7.31 (DNA Nucleotidylexotransferase)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Cell Adhesion
MH  - Cell Death
MH  - DNA Nucleotidylexotransferase
MH  - Deoxyuracil Nucleotides
MH  - Epithelium/physiology/surgery/ultrastructure
MH  - Female
MH  - Lens, Crystalline/*physiology/*surgery/ultrastructure
MH  - Necrosis
MH  - Rabbits
MH  - Wound Healing
EDAT- 1997/10/31 00:00
MHDA- 1997/10/31 00:01
CRDT- 1997/10/31 00:00
PHST- 1997/10/31 00:00 [pubmed]
PHST- 1997/10/31 00:01 [medline]
PHST- 1997/10/31 00:00 [entrez]
PST - ppublish
SO  - Invest Ophthalmol Vis Sci. 1997 Oct;38(11):2322-30.