PMID- 9347321
OWN - NLM
STAT- MEDLINE
DCOM- 19971204
LR  - 20190826
IS  - 0306-3623 (Print)
IS  - 0306-3623 (Linking)
VI  - 29
IP  - 5
DP  - 1997 Nov
TI  - Inhibition of muscarinic receptor-operated Ca2+ sensitization by short-term 
      desensitization of alpha-toxin-permeabilized smooth muscle cells from guinea pig 
      stomach.
PG  - 753-61
AB  - 1. Isolated single smooth muscle cells from the guinea pig stomach were 
      permeabilized with Staphylococcus aureus alpha-toxin. 2. The permeabilized single 
      cells showed a shortening in response to Ca2+ in an all-or-none manner. Moreover, 
      the addition of acetylcholine (ACh) or guanosine 5'-triphosphate (GTP) resulted 
      in a decrease in concentration of Ca2+ required to trigger a threshold response, 
      suggesting that Ca2+ sensitization is induced by the stimulation of muscarinic 
      acetylcholine receptors (mAChRs) or GTP-binding protein(s). 3. Short-term 
      desensitization was induced by incubating the permeabilized cells with 100 microM 
      ACh for 10 min. 4. In desensitized cells, the concentration of Ca2+ required to 
      trigger a threshold response in the presence of ACh was increased, however, the 
      cell shortening in response to Ca2+ in the absence of ACh and GTP-induced Ca2+ 
      sensitization was not affected by short-term desensitization. 5. These results 
      suggest that the receptor-operated augmentation of Ca2+ sensitivity is inhibited 
      by short-term desensitization and that the development of short-term 
      desensitization is due to an uncoupling of mAChR/GTP-binding protein(s).
FAU - Mita, M
AU  - Mita M
AD  - Department of Pharmacology, Meiji College of Pharmacy, Tokyo, Japan.
FAU - Satoh-Akimoto, K
AU  - Satoh-Akimoto K
FAU - Oishi, K
AU  - Oishi K
FAU - Hashimoto, T
AU  - Hashimoto T
FAU - Uchida, M K
AU  - Uchida MK
LA  - eng
PT  - Journal Article
PL  - England
TA  - Gen Pharmacol
JT  - General pharmacology
JID - 7602417
RN  - 0 (Muscarinic Antagonists)
RN  - 0 (Receptors, Muscarinic)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - N9YNS0M02X (Acetylcholine)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Acetylcholine/pharmacology
MH  - Animals
MH  - Calcium/*pharmacology
MH  - Cell Membrane Permeability/drug effects
MH  - Gastric Fundus/cytology/drug effects
MH  - Guanosine Triphosphate/pharmacology
MH  - Guinea Pigs
MH  - In Vitro Techniques
MH  - Muscarinic Antagonists/*pharmacology
MH  - Muscle Contraction/drug effects
MH  - Muscle, Smooth/cytology/*drug effects
MH  - Receptors, Muscarinic/*metabolism
MH  - Stomach/cytology/*drug effects
MH  - Type C Phospholipases/*pharmacology
EDAT- 1997/11/05 00:00
MHDA- 1997/11/05 00:01
CRDT- 1997/11/05 00:00
PHST- 1997/11/05 00:00 [pubmed]
PHST- 1997/11/05 00:01 [medline]
PHST- 1997/11/05 00:00 [entrez]
AID - S0306362397000335 [pii]
AID - 10.1016/s0306-3623(97)00033-5 [doi]
PST - ppublish
SO  - Gen Pharmacol. 1997 Nov;29(5):753-61. doi: 10.1016/s0306-3623(97)00033-5.