PMID- 9348186
OWN - NLM
STAT- MEDLINE
DCOM- 19971124
LR  - 20220331
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 138
IP  - 11
DP  - 1997 Nov
TI  - Tissue distribution and quantitative analysis of estrogen receptor-alpha 
      (ERalpha) and estrogen receptor-beta (ERbeta) messenger ribonucleic acid in the 
      wild-type and ERalpha-knockout mouse.
PG  - 4613-21
AB  - Until recently, only a single type of estrogen receptor (ER) was thought to exist 
      and mediate the genomic effects of the hormone 17beta-estradiol in mammalian 
      tissues. However, the cloning of a gene encoding a second type of ER, termed 
      ERbeta, from the mouse, rat, and human has prompted a reevaluation of the 
      estrogen signaling system. Based on in vitro studies, the ERbeta protein binds 
      estradiol with an affinity similar to that of the classical ER (now referred to 
      as ERalpha) and is able to mediate the effects of estradiol in transfected 
      mammalian cell lines. Essential to further investigations of the possible 
      physiological roles of ERbeta, and its possible interactions with ERalpha, are 
      data on the tissue distribution of the two ER types. Herein, we have described 
      the optimization and use of an RNase protection assay able to detect and 
      distinguish messenger RNA (mRNA) transcripts from both the ERalpha and ERbeta 
      genes in the mouse. Because this assay is directly quantitative, a comparison of 
      the levels of expression within various tissues was possible. In addition, the 
      effect of disruption of the ERalpha gene on the expression of the ERbeta gene was 
      also investigated using the ERalpha-knockout (ERKO) mouse. Transcripts encoding 
      ERalpha were detected in all the wild-type tissues assayed from both sexes. In 
      the female reproductive tract, the highest expression of ERbeta mRNA was observed 
      in the ovary and showed great variation among individual animals; detectable 
      levels were observed in the uterus and oviduct, whereas mammary tissue was 
      negative. In the male reproductive tract, significant expression of ERbeta was 
      seen in the prostate and epididymis, whereas the testes were negative. In other 
      tissues of both sexes, the hypothalamus and lung were clearly positive for both 
      ERalpha and ERbeta mRNA. The ERKO mice demonstrated slightly reduced levels of 
      ERbeta mRNA in the ovary, prostate, and epididymis. These data, in combination 
      with the several described phenotypes in both sexes of the ERKO mouse, suggest 
      that the biological functions of the ERbeta protein may be dependent on the 
      presence of ERalpha in certain cell types and tissues. Further characterization 
      of the physiological phenotypes in the ERKO mice may elucidate possible ERbeta 
      specific actions.
FAU - Couse, J F
AU  - Couse JF
AD  - Laboratory of Reproductive and Developmental Toxicology, National Institute of 
      Environmental Health Sciences, National Institutes of Health, Research Triangle 
      Park, North Carolina 27709, USA.
FAU - Lindzey, J
AU  - Lindzey J
FAU - Grandien, K
AU  - Grandien K
FAU - Gustafsson, J A
AU  - Gustafsson JA
FAU - Korach, K S
AU  - Korach KS
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Estrogen Receptor beta)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Estrogen)
RN  - EC 3.1.- (Ribonucleases)
SB  - IM
MH  - Animals
MH  - Brain/metabolism
MH  - Estrogen Receptor alpha
MH  - Estrogen Receptor beta
MH  - Female
MH  - Genitalia, Female/metabolism
MH  - Genitalia, Male/metabolism
MH  - Male
MH  - Mammary Glands, Animal/metabolism
MH  - Mice
MH  - Mice, Knockout/genetics
MH  - Nucleic Acid Hybridization
MH  - Pituitary Gland/metabolism
MH  - RNA, Messenger/*metabolism
MH  - Receptors, Estrogen/*genetics
MH  - Ribonucleases
MH  - Tissue Distribution
EDAT- 1997/11/05 00:00
MHDA- 1997/11/05 00:01
CRDT- 1997/11/05 00:00
PHST- 1997/11/05 00:00 [pubmed]
PHST- 1997/11/05 00:01 [medline]
PHST- 1997/11/05 00:00 [entrez]
AID - 10.1210/endo.138.11.5496 [doi]
PST - ppublish
SO  - Endocrinology. 1997 Nov;138(11):4613-21. doi: 10.1210/endo.138.11.5496.