PMID- 9348244
OWN - NLM
STAT- MEDLINE
DCOM- 19971124
LR  - 20131121
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 138
IP  - 11
DP  - 1997 Nov
TI  - Quantitative analysis of growth hormone (GH) pre-mRNA expression in cultured rat 
      anterior pituitary cells by an intron-specific and competitive PCR method.
PG  - 5075-8
AB  - We have developed a novel method of quantifying growth hormone(GH) pre-mRNA 
      expression in anterior pituitary cells. DNA-free total RNA extracted from 
      cultured rat anterior pituitary cells was reverse transcribed(RT) to cDNA, and RT 
      products were subsequently quantitated by competitive PCR using intron-specific 
      primers of rat GH gene. After 6-h of incubation in treated cells, 
      dexamethasone(Dex) and triiodo-L-thyronine(T3) significantly increased GH 
      pre-mRNA levels(3.2- and 2.2-fold compared to non-treated cells, respectively). 
      However, Northern blot analysis did not detect significant changes in GH mRNA 
      levels. After 24-h incubation with Dex and T3, significant increases in GH mRNA 
      levels were detected on Northern blots, but GH pre-mRNA levels did not differ 
      between treated and non-treated cells. These findings suggest that both Dex and 
      T3 treatments rapidly increase GH pre-mRNA levels in normal somatotropes. This 
      method has high sensitivity and widespread application to the analysis of 
      pre-mRNAs of target genes.
FAU - Matsubara, S
AU  - Matsubara S
AD  - First Department of Internal Medicine, Kagawa Medical University, Japan.
FAU - Sato, M
AU  - Sato M
FAU - Ohye, H
AU  - Ohye H
FAU - Murao, K
AU  - Murao K
FAU - Takahara, J
AU  - Takahara J
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Glucocorticoids)
RN  - 0 (RNA Precursors)
RN  - 0 (RNA, Messenger)
RN  - 06LU7C9H1V (Triiodothyronine)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - 9002-72-6 (Growth Hormone)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Dexamethasone/pharmacology
MH  - Glucocorticoids/pharmacology
MH  - Growth Hormone/*genetics
MH  - Introns/genetics
MH  - Male
MH  - Pituitary Gland, Anterior/cytology/*metabolism
MH  - Polymerase Chain Reaction/methods
MH  - RNA Precursors/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Time Factors
MH  - Transcription, Genetic
MH  - Triiodothyronine/pharmacology
EDAT- 1997/11/05 00:00
MHDA- 1997/11/05 00:01
CRDT- 1997/11/05 00:00
PHST- 1997/11/05 00:00 [pubmed]
PHST- 1997/11/05 00:01 [medline]
PHST- 1997/11/05 00:00 [entrez]
AID - 10.1210/endo.138.11.5654 [doi]
PST - ppublish
SO  - Endocrinology. 1997 Nov;138(11):5075-8. doi: 10.1210/endo.138.11.5654.