PMID- 9349510
OWN - NLM
STAT- MEDLINE
DCOM- 19971113
LR  - 20061115
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 15
IP  - 14
DP  - 1997 Oct 2
TI  - Induction of telomerase activity by UV-irradiation in Chinese hamster cells.
PG  - 1747-52
AB  - Telomerase is a ribonucleoprotein whose activity has been detected in germline 
      cells and in neoplastic and immortal cells. Telomerase compensates the telomere 
      loss arising by the end replication problem by synthesizing telomeric repeats at 
      the 3' end of the eukaryotic chromosomes. Telomerase is reactivated during cancer 
      progression in human and mice. In order to determine whether the telomerase 
      activity can be upregulated in vitro in response to DNA damaging agents, we 
      examined the telomerase activity in five Chinese hamster cell lines following 
      exposure to 5 J/m2 or 40 J/m2 UV-C radiation. All the cell lines tested showed an 
      increase in telomerase activity in the PCR-based telomeric repeat amplification 
      protocol (TRAP) in a dose dependent manner. This increase in telomerase activity 
      correlated well with the number of cells being in the S and G2/M phase after UV 
      exposure. However, in unirradiated control cells, similar levels of telomerase 
      activity were observed in different phases of the cell cycle. Furthermore, 
      telomeric signals were clustered in one or more parts of the disintegrating 
      nuclear particles of the apoptotic cell as detected by fluorescence in situ 
      hybridization (FISH). This is the first study to demonstrate the induction of 
      telomerase activity following exposure to DNA-damaging agents like UV radiation 
      in Chinese hamster cells in vitro.
FAU - Hande, M P
AU  - Hande MP
AD  - MGC-Department of Radiation Genetics and Chemical Mutagenesis, Leiden University, 
      The Netherlands.
FAU - Balajee, A S
AU  - Balajee AS
FAU - Natarajan, A T
AU  - Natarajan AT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Animals
MH  - CHO Cells
MH  - Cell Cycle
MH  - Cricetinae
MH  - DNA Damage/radiation effects
MH  - Enzyme Induction/radiation effects
MH  - In Situ Hybridization, Fluorescence
MH  - Telomerase/*metabolism
MH  - Telomere/metabolism
MH  - Ultraviolet Rays
EDAT- 1998/02/12 00:00
MHDA- 1998/02/12 00:01
CRDT- 1998/02/12 00:00
PHST- 1998/02/12 00:00 [pubmed]
PHST- 1998/02/12 00:01 [medline]
PHST- 1998/02/12 00:00 [entrez]
AID - 10.1038/sj.onc.1201327 [doi]
PST - ppublish
SO  - Oncogene. 1997 Oct 2;15(14):1747-52. doi: 10.1038/sj.onc.1201327.