PMID- 9349518
OWN - NLM
STAT- MEDLINE
DCOM- 19971104
LR  - 20190620
IS  - 0008-543X (Print)
IS  - 0008-543X (Linking)
VI  - 81
IP  - 5
DP  - 1997 Oct 25
TI  - Detection of hyperdiploid malignant cells in body cavity effusions by fluoresence 
      in situ hybridization on ThinPrep slides.
PG  - 299-308
AB  - BACKGROUND: Benign body cavity effusions sometimes cannot be distinguished from 
      malignant ones by conventional cytology. The authors performed fluorescence in 
      situ hybridization (FISH) on ThinPrep slides using chromosome specific probes to 
      see if hyperdiploid malignant cells could be detected in 20 body cavity 
      effusions. The results were then compared with those of conventional cytology. 
      METHODS: A total of 20 body cavity effusions from 19 patients were studied using 
      conventional cytology and FISH. Probes specific for chromosomes 3, 8, 10, and 12 
      were used to detect hyperdiploidy on ThinPrep slides (Cytyc Corporation, 
      Boxborough, MA). RESULTS: A total of 13 patients had malignant conditions (either 
      prior history of malignancy or the presence of malignancy anywhere in the body). 
      Conventional cytology and FISH were both positive in 5 of these patients (6 
      samples) and negative in 2 patients. The results for one sample were inconclusive 
      by both methods. There were 5 discrepant cytology-FISH results in patients with 
      malignant conditions. One sample was positive by FISH and negative by cytology, 
      one was positive by FISH and "atypical" by cytology, and three were inconclusive 
      by FISH and negative by cytology. FISH results were either negative (in 4 
      samples) or inconclusive (in 2 samples) in the 6 patients with benign conditions. 
      CONCLUSIONS: FISH can detect hyperdiploid malignant cells in body cavity 
      effusions and is especially useful when the major cell population consists of 
      malignant cells that cannot be differentiated from mesothelial or "atypical" 
      cells. It is less useful in detecting a small population of malignant cells 
      hidden in an inflammatory or reactive cell background. More studies are needed to 
      establish diagnostic criteria further and to assess the clinical usefulness of 
      this procedure.
FAU - Florentine, B D
AU  - Florentine BD
AD  - Los Angeles County-University of Southern California Medical Center, Los Angeles 
      90033, USA.
FAU - Sanchez, B
AU  - Sanchez B
FAU - Raza, A
AU  - Raza A
FAU - Frankel, K
AU  - Frankel K
FAU - Martin, S E
AU  - Martin SE
FAU - Kovacs, B
AU  - Kovacs B
FAU - Felix, J C
AU  - Felix JC
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Cancer
JT  - Cancer
JID - 0374236
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Ascitic Fluid/metabolism/*pathology
MH  - Chromosomes, Human, Pair 10/metabolism
MH  - Chromosomes, Human, Pair 12/metabolism
MH  - Chromosomes, Human, Pair 3/metabolism
MH  - Chromosomes, Human, Pair 8/metabolism
MH  - *Diploidy
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Neoplasms/metabolism/*pathology
MH  - Pericardial Effusion/metabolism/*pathology
MH  - Pleural Effusion, Malignant/metabolism/*pathology
EDAT- 1998/02/12 04:51
MHDA- 2000/06/20 09:00
CRDT- 1998/02/12 04:51
PHST- 1998/02/12 04:51 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1998/02/12 04:51 [entrez]
AID - 10.1002/(SICI)1097-0142(19971025)81:5<299::AID-CNCR8>3.0.CO;2-I [pii]
AID - 10.1002/(sici)1097-0142(19971025)81:5<299::aid-cncr8>3.0.co;2-i [doi]
PST - ppublish
SO  - Cancer. 1997 Oct 25;81(5):299-308. doi: 
      10.1002/(sici)1097-0142(19971025)81:5<299::aid-cncr8>3.0.co;2-i.