PMID- 9350650
OWN - NLM
STAT- MEDLINE
DCOM- 19971201
LR  - 20190725
IS  - 0085-2538 (Print)
IS  - 0085-2538 (Linking)
VI  - 52
IP  - 5
DP  - 1997 Nov
TI  - Effect of glycine on prelethal and postlethal increases in calpain activity in 
      rat renal proximal tubules.
PG  - 1271-8
AB  - The effect of glycine on hypoxia- and ionomycin-induced increases in calpain 
      activity in rat proximal tubules was determined. Calpain activity was determined 
      both in vitro and in the intact cell using the fluorescent substrate 
      N-succinyl-Leu-Leu-Val-Tyr-7-amido-4-methyl coumarin 
      (N-succinyl-Leu-Leu-Val-Tyr-AMC) and Western blotting for calpain-specific 
      spectrin breakdown products (BDP), respectively. At 7.5 minutes of hypoxia 
      (prelethal injury model) there was a significant (10-fold) increase in in vitro 
      calpain activity that was not inhibited by glycine. At 15 minutes of hypoxia 
      (postlethal injury model) there was a further increase in calpain activity that 
      was inhibited by glycine. Normoxic tubules incubated with the calcium ionophore 
      ionomycin (5 microM) for two minutes and 10 minutes had a significant increase in 
      calpain activity that was not inhibited by glycine. After 15 minutes of hypoxia 
      in the presence of glycine, there was an increase in calpain-specific spectrin 
      breakdown products (BDP) in both Triton X-100 soluble and cytosolic extracts from 
      proximal tubules. Glycine in concentrations up to 10 mM had no direct effect on 
      the in vitro calpain activity of purified calpains. The present study 
      demonstrates that: (1) prelethal increases in calpain activity stimulated by 
      hypoxia and ionomycin treatment are not affected by glycine; (2) calpain-mediated 
      spectrin breakdown during hypoxia occurs in the presence of glycine; (3) glycine 
      does prevent the additional postlethal increase in calpain activity probably by 
      maintaining membrane integrity to calcium influx.
FAU - Edelstein, C L
AU  - Edelstein CL
AD  - Department of Medicine, University of Colorado School of Medicine, Denver, USA.
FAU - Ling, H
AU  - Ling H
FAU - Gengaro, P E
AU  - Gengaro PE
FAU - Nemenoff, R A
AU  - Nemenoff RA
FAU - Bahr, B A
AU  - Bahr BA
FAU - Schrier, R W
AU  - Schrier RW
LA  - eng
GR  - DK35098/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Kidney Int
JT  - Kidney international
JID - 0323470
RN  - 12634-43-4 (Spectrin)
RN  - 56092-81-0 (Ionomycin)
RN  - EC 3.4.22.- (Calpain)
RN  - SY7Q814VUP (Calcium)
RN  - TE7660XO1C (Glycine)
SB  - IM
MH  - Animals
MH  - Calcium/metabolism
MH  - Calpain/antagonists & inhibitors/*metabolism
MH  - Cytoprotection
MH  - Glycine/*pharmacology
MH  - Ionomycin/pharmacology
MH  - Kidney Tubules, Proximal/*drug effects/enzymology
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spectrin/metabolism
EDAT- 1997/11/14 00:00
MHDA- 1997/11/14 00:01
CRDT- 1997/11/14 00:00
PHST- 1997/11/14 00:00 [pubmed]
PHST- 1997/11/14 00:01 [medline]
PHST- 1997/11/14 00:00 [entrez]
AID - S0085-2538(15)60292-7 [pii]
AID - 10.1038/ki.1997.452 [doi]
PST - ppublish
SO  - Kidney Int. 1997 Nov;52(5):1271-8. doi: 10.1038/ki.1997.452.