PMID- 9350749
OWN - NLM
STAT- MEDLINE
DCOM- 19980203
LR  - 20210526
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 35
IP  - 11
DP  - 1997 Nov
TI  - Molecular evidence and clinical significance of herpesvirus coinfection in the 
      central nervous system.
PG  - 2869-72
AB  - A total of 60 cerebrospinal fluid (CSF) specimens from patients manifesting 
      symptoms resembling viral central nervous system (CNS) disease were examined for 
      the presence of herpes simplex virus (HSV), human herpesvirus 6 (HHV-6), 
      Epstein-Barr virus (EBV), cytomegalovirus, varicella-zoster virus, Borrelia 
      burgdorferi, and Tropheryma whippelii DNA by PCR. Of 30 specimens which were 
      selected on the basis of HSV DNA positivity, 2 were concomitantly positive for 
      HHV-6 DNA and 1 was positive for EBV DNA. In the three specimens positive for 
      more than one herpesvirus, amplicons generated with virus-specific primer sets 
      hybridized specifically to the corresponding virus-specific probe. Sequence 
      analysis of the two amplified DNA fragments demonstrated that they were derived 
      from distinct herpesviruses. Of 22 patients with clinically diagnosed 
      encephalitis, 2 of 3 patients coinfected with HSV and HHV-6 died, compared to 1 
      of 19 (5%) patients infected with only HSV. Of 30 CSF specimens that were 
      negative for HSV DNA, EBV DNA was detected in one sample. These data indicated 
      the presence of DNA specific for two distinct herpesviruses in the same CSF 
      specimen, providing molecular evidence that coinfection with this group of 
      viruses may occur in the CNS.
FAU - Tang, Y W
AU  - Tang YW
AD  - Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, 
      Minnesota 55905, USA.
FAU - Espy, M J
AU  - Espy MJ
FAU - Persing, D H
AU  - Persing DH
FAU - Smith, T F
AU  - Smith TF
LA  - eng
GR  - AI-30548/AI/NIAID NIH HHS/United States
GR  - AI-32403/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Viral)
RN  - 0 (Oligonucleotide Probes)
SB  - IM
MH  - Borrelia burgdorferi Group/isolation & purification
MH  - Central Nervous System Diseases/*cerebrospinal fluid/microbiology/*virology
MH  - Cytomegalovirus/isolation & purification
MH  - DNA Primers
MH  - DNA, Viral/cerebrospinal fluid
MH  - Encephalitis, Viral/cerebrospinal fluid/*diagnosis/mortality
MH  - Enterococcus/isolation & purification
MH  - Herpes Simplex/cerebrospinal fluid/complications/diagnosis
MH  - Herpesviridae Infections/cerebrospinal fluid/*complications/diagnosis
MH  - Herpesvirus 3, Human/isolation & purification
MH  - Herpesvirus 4, Human/isolation & purification
MH  - Herpesvirus 6, Human/isolation & purification
MH  - Humans
MH  - Oligonucleotide Probes
MH  - Polymerase Chain Reaction/*methods
MH  - Sensitivity and Specificity
MH  - Simplexvirus/*isolation & purification
MH  - Survival Rate
PMC - PMC230077
EDAT- 1997/11/14 00:00
MHDA- 1997/11/14 00:01
PMCR- 1997/11/01
CRDT- 1997/11/14 00:00
PHST- 1997/11/14 00:00 [pubmed]
PHST- 1997/11/14 00:01 [medline]
PHST- 1997/11/14 00:00 [entrez]
PHST- 1997/11/01 00:00 [pmc-release]
AID - 10.1128/jcm.35.11.2869-2872.1997 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1997 Nov;35(11):2869-72. doi: 10.1128/jcm.35.11.2869-2872.1997.