PMID- 9350767
OWN - NLM
STAT- MEDLINE
DCOM- 19980203
LR  - 20211216
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 35
IP  - 11
DP  - 1997 Nov
TI  - Genes coding for enterotoxins and verotoxins in porcine Escherichia coli strains 
      belonging to different O:K:H serotypes: relationship with toxic phenotypes.
PG  - 2958-63
AB  - Seventy-four E. coli strains isolated from piglets with diarrhea or edema disease 
      in Spain were serotyped and examined for production of heat-labile (LT) and 
      heat-stable (ST) enterotoxins (LT-I, LT-II, STaH, STaP, and STb) and verotoxins 
      (VT1, VT2, and VT2v = VTe) by phenotypic (Vero cell assay and infant mouse test) 
      and genotypic (colony hybridization and PCR) methods. In general, an excellent 
      correlation was found between the results obtained with a PCR approach and those 
      determined with biological assays. DNA probes used in the hybridization also 
      showed a very good agreement with phenotypic results, with the exception of a VT1 
      probe that initially produced 10 false-positive reactions. The gene coding for 
      STb (58 strains) was the most prevalent gene detected by PCR, followed by those 
      coding for STa (46 strains), LT (19 strains), VT2v (11 strains), and VT1 (1 
      strain). Apparently, in Spain three seropathotypes are predominant: (i) 
      O149:K91:H10 K88+ LT-I+ STb+, (ii) O141:K85ab:H- P987+ STaP+, and (iii) 
      O138:K81:H14 or H- STaP+ VT2v+. We conclude that PCR is a fast, specific, and 
      practical method for the identification of enterotoxin and VT genes in clinical 
      and epidemiological studies.
FAU - Blanco, M
AU  - Blanco M
AD  - Departamento de Microbiologia y Parasitologia, Facultad de Veterinaria, 
      Universidad de Santiago de Compostela, Lugo, Spain.
FAU - Blanco, J E
AU  - Blanco JE
FAU - Gonzalez, E A
AU  - Gonzalez EA
FAU - Mora, A
AU  - Mora A
FAU - Jansen, W
AU  - Jansen W
FAU - Gomes, T A
AU  - Gomes TA
FAU - Zerbini, L F
AU  - Zerbini LF
FAU - Yano, T
AU  - Yano T
FAU - de Castro, A F
AU  - de Castro AF
FAU - Blanco, J
AU  - Blanco J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Bacterial Toxins)
RN  - 0 (Enterotoxins)
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (Shiga Toxin 1)
RN  - 0 (Shiga Toxin 2)
RN  - 0 (heat stable toxin (E coli))
RN  - D9K3SN2LNY (heat-labile enterotoxin, E coli)
SB  - IM
MH  - Animals
MH  - Bacterial Toxins/biosynthesis
MH  - Chlorocebus aethiops
MH  - Diarrhea/microbiology/veterinary
MH  - Edema
MH  - Enterotoxins/*biosynthesis
MH  - Escherichia coli/classification/*genetics/physiology
MH  - Escherichia coli Infections/microbiology/*veterinary
MH  - *Escherichia coli Proteins
MH  - Genes, Bacterial
MH  - Genotype
MH  - Mice
MH  - Phenotype
MH  - Serotyping
MH  - Shiga Toxin 1
MH  - Shiga Toxin 2
MH  - Swine
MH  - *Swine Diseases
MH  - Vero Cells
PMC - PMC230095
EDAT- 1997/11/14 00:00
MHDA- 1997/11/14 00:01
PMCR- 1997/11/01
CRDT- 1997/11/14 00:00
PHST- 1997/11/14 00:00 [pubmed]
PHST- 1997/11/14 00:01 [medline]
PHST- 1997/11/14 00:00 [entrez]
PHST- 1997/11/01 00:00 [pmc-release]
AID - 10.1128/jcm.35.11.2958-2963.1997 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1997 Nov;35(11):2958-63. doi: 10.1128/jcm.35.11.2958-2963.1997.