PMID- 9352327
OWN - NLM
STAT- MEDLINE
DCOM- 19971218
LR  - 20191102
IS  - 1269-3286 (Print)
IS  - 1269-3286 (Linking)
VI  - 39
IP  - 4
DP  - 1997 Aug
TI  - Cytokine mediated expansion of human umbilical cord blood CD34+ cells: comparison 
      of the use of partially purified and pure CD34+ target cells.
PG  - 193-7
AB  - To determine the optimal cell population for cytokine mediated expansion, we 
      compared the use of Magnetic Cell Sorting (MACS) system enriched CD34+ human 
      umbilical cord blood (HUCB) cells with that of MACS enriched, flow purified CD34+ 
      HUCB cells. Both MACS enriched CD34+ cells and MACS enriched, flow purified CD34+ 
      cells (mean starting purity of CD34+ SC 51.27 +/- 7.6% and 96.36 +/- 1.34% 
      respectively n = 6) were incubated for seven days with Interleukin-1 (IL-1) + 
      IL-3 + Stem Cell Factor (SCF) and showed a fold increase in the number of 
      nucleated cells (10.02 +/- 2.6 and 18.23 +/- 4.73 respectively) and a reduction 
      in the percentage of CD34+ cells (5.55 +/- 1.23% and 12.21 +/- 3.29% 
      respectively). An increase in the absolute numbers of CD34+ cells (4.8 x 10(4) 
      +/- 2.3 x 10(4)) was observed with MACS enriched CD34+ cells as compared to no 
      change (1.3 x 10(5) +/- 8.8 x 10(4) with MACS enriched, flow purified CD34+ 
      cells. An increase in IL-3 + GM-CSF + SCF responsive colony forming unit (CFU) 
      (1.7 x 10(4) +/- 9.4 x 10(3) and 1.6 x 10(5) +/- 7.7 x 10(4) respectively) was 
      also observed as compared with input values (1.5 x 10(4) +/- 1 x 10(4) and 2.3 x 
      10(4) +/- 8.9 x 10(3) respectively). We conclude that MACS enriched, flow sorted 
      CD34+ HUCB cells have greater cytokine mediated expansion potential as measured 
      by progenitor expansion, than MACS enriched CD34+ HUCB cells.
FAU - Case, J
AU  - Case J
AD  - Bone Marrow Transplant Laboratory, Children's Leukaemia & Cancer Research Centre, 
      Sydney Children's Hospital, Randwick, NSW, Australia.
FAU - Rice, A
AU  - Rice A
FAU - Vowels, M
AU  - Vowels M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Hematol Cell Ther
JT  - Hematology and cell therapy
JID - 9613253
RN  - 0 (Antigens, CD34)
RN  - 0 (Cytokines)
SB  - IM
MH  - Antigens, CD34/*blood
MH  - Cell Count
MH  - Cell Nucleus
MH  - Cell Separation/methods
MH  - Cytokines/*physiology
MH  - Fetal Blood/cytology/*immunology
MH  - Hematopoietic Stem Cells/*immunology
MH  - Humans
MH  - Magnetics
EDAT- 1997/08/01 00:00
MHDA- 1997/11/14 00:01
CRDT- 1997/08/01 00:00
PHST- 1997/08/01 00:00 [pubmed]
PHST- 1997/11/14 00:01 [medline]
PHST- 1997/08/01 00:00 [entrez]
AID - 10.1007/s00282-997-0193-2 [doi]
PST - ppublish
SO  - Hematol Cell Ther. 1997 Aug;39(4):193-7. doi: 10.1007/s00282-997-0193-2.