PMID- 9352440
OWN - NLM
STAT- MEDLINE
DCOM- 19971222
LR  - 20200409
IS  - 0021-9975 (Print)
IS  - 1532-3129 (Electronic)
IS  - 0021-9975 (Linking)
VI  - 117
IP  - 2
DP  - 1997 Aug
TI  - Detection of nucleic acids of porcine reproductive and respiratory syndrome virus 
      in the lungs of naturally infected piglets as determined by in-situ 
      hybridization.
PG  - 157-63
AB  - Replication of porcine reproductive and respiratory syndrome virus (PRRSV) was 
      studied in formalin-fixed paraffin wax-embedded lung tissues from seven naturally 
      infected piglets by in-situ hybridization with a non-radioactive 
      digoxigenin-labelled probe. A 433 base pair cDNA probe for the viral RNA encoding 
      the nucleocapsid proteins of a Korean PRRSV isolate was generated by the 
      polymerase chain reaction. All seven piglets infected with PRRSV showed a 
      distinct, positive signal, scattered throughout the alveolar septa and spaces. 
      Positive cells typically exhibited dark brown staining deposits in the cytoplasm 
      without background staining. In-situ hybridization demonstrated that PRRSV 
      replicated primarily in interstitial and alveolar macrophages, and occasionally 
      in type 2 pneumocytes. The bronchial or bronchiolar epithelium did not exhibit a 
      hybridization signal for PRRSV nucleic acids. The anterior and middle lobes of 
      the lung were more reliable than the caudal or accessory lobes for the detection 
      of PRRSV nucleic acids. The in-situ hybridization technique used was rapid, 
      specific and sensitive, and may prove useful for the diagnosis of PRRSV infection 
      in routinely fixed and processed tissues.
FAU - Cheon, D S
AU  - Cheon DS
AD  - Department of Veterinary Pathology, College of Veterinary Medicine, Seoul 
      National University, Kyounggi-Do, Republic of Korea.
FAU - Chae, C
AU  - Chae C
FAU - Lee, Y S
AU  - Lee YS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Comp Pathol
JT  - Journal of comparative pathology
JID - 0102444
RN  - 0 (Nucleic Acids)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - In Situ Hybridization
MH  - Lung/*chemistry/pathology
MH  - Nucleic Acids/*analysis
MH  - Porcine Reproductive and Respiratory Syndrome/*genetics/pathology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics
MH  - RNA, Viral/analysis
MH  - Swine
MH  - Swine Diseases/*genetics/pathology
PMC - PMC7130319
EDAT- 1997/08/01 00:00
MHDA- 1997/11/14 00:01
PMCR- 2006/06/06
CRDT- 1997/08/01 00:00
PHST- 1997/08/01 00:00 [pubmed]
PHST- 1997/11/14 00:01 [medline]
PHST- 1997/08/01 00:00 [entrez]
PHST- 2006/06/06 00:00 [pmc-release]
AID - S0021-9975(97)80032-1 [pii]
AID - 10.1016/s0021-9975(97)80032-1 [doi]
PST - ppublish
SO  - J Comp Pathol. 1997 Aug;117(2):157-63. doi: 10.1016/s0021-9975(97)80032-1.