PMID- 9352644
OWN - NLM
STAT- MEDLINE
DCOM- 19980202
LR  - 20190914
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 40
IP  - 5
DP  - 1997 Oct
TI  - Physical localisation of repetitive DNA sequences in Alstroemeria: karyotyping of 
      two species with species-specific and ribosomal DNA.
PG  - 652-8
AB  - Fluorescence in situ hybridization (FISH) was used to localise two 
      species-specific repetitive DNA sequences, A001-I and D32-13, and two highly 
      conserved 25S and 5S rDNA sequences on the metaphase chromosomes of two species 
      of Alstroemeria. The Chilean species, Alstroemeria aurea (2n = 16), has abundant 
      constitutive heterochromatin, whereas the Brazilian species, Alstroemeria 
      inodora, has hardly any heterochromatin. The A. aurea specific A001-I probe 
      hybridized specifically to the C-band regions on all chromosomes. The FISH 
      patterns on A. inodora chromosomes using species-specific probe D32-13 resembled 
      the C-banding pattern and the A001-I pattern on A. aurea chromosomes. There were 
      notable differences in number and distribution of rDNA sites between the two 
      species. The 25S rDNA probe revealed 16 sites in A. aurea that closely 
      colocalised with A001-I sites and 12 in A. inodora that were predominantly 
      detected in the centromeric regions. FISH karyotypes of the two Alstroemeria 
      species were constructed accordingly, enabling full identification of all 
      individual chromosomes. These FISH karyotypes will be useful for monitoring the 
      chromosomes of both Alstroemeria species in hybrids and backcross derivatives.
FAU - Kamstra, S A
AU  - Kamstra SA
AD  - Department of Plant Breeding, Graduate School of Experimental Plant Sciences, 
      Wageningen Agricultural University, The Netherlands. 
      -ilvan.Kamstra@users.pv.wau.nl
FAU - Kuipers, A G
AU  - Kuipers AG
FAU - De Jeu, M J
AU  - De Jeu MJ
FAU - Ramanna, M S
AU  - Ramanna MS
FAU - Jacobsen, E
AU  - Jacobsen E
LA  - eng
PT  - Journal Article
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
RN  - 0 (DNA, Plant)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Indoles)
RN  - 47165-04-8 (DAPI)
SB  - IM
MH  - Brazil
MH  - Chile
MH  - Chromosomes/chemistry
MH  - DNA, Plant/*analysis
MH  - DNA, Ribosomal/*analysis
MH  - Fluorescent Dyes/metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Indoles/metabolism
MH  - Karyotyping
MH  - Plants/*genetics
MH  - *Repetitive Sequences, Nucleic Acid
MH  - Species Specificity
EDAT- 1997/11/14 00:00
MHDA- 1997/11/14 00:01
CRDT- 1997/11/14 00:00
PHST- 1997/11/14 00:00 [pubmed]
PHST- 1997/11/14 00:01 [medline]
PHST- 1997/11/14 00:00 [entrez]
AID - 10.1139/g97-086 [doi]
PST - ppublish
SO  - Genome. 1997 Oct;40(5):652-8. doi: 10.1139/g97-086.