PMID- 9352794
OWN - NLM
STAT- MEDLINE
DCOM- 19971124
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 99
IP  - 1
DP  - 1997 Nov
TI  - Chromosome aberrations in renal tumors detected by fluorescence in situ 
      hybridization.
PG  - 38-44
AB  - The aim of this study was to investigate the relationship between chromosome 
      aberrations detected by fluorescence in situ hybridization (FISH) and tumor 
      grade, stage, venous involvement, and DNA ploidy status in 18 renal tumors. Using 
      FISH with chromosome-specific DNA probes, the copy number of pericentromeric 
      sequences on chromosomes 3, 7, 9, and 17 was detected within interphase nuclei in 
      touch preparations from tumor specimens. Monosomy for chromosome 3 was detected 
      in seven of 9 DNA diploid tumors, whereas all DNA aneuploid tumors demonstrated 
      trisomy or tetrasomy for chromosome 7. Moreover, monosomy for chromosome 3 was 
      more frequently shown in the diploid and low-stage tumors than in the aneuploid 
      and high-stage tumors. The percentage of hyperdiploid cells significantly 
      correlated with DNA ploidy status in the case of chromosomes 3 and 7 (p = 0.030, 
      p = 0.007, respectively). The percentage of hyperdiploid cells for chromosome 3 
      had borderline significance with tumor stage. On the other hand, the percentage 
      of diploid cells for chromosome 17 was significantly correlated with DNA ploidy 
      status and tumor stage (p = 0.030, p = 0.027, respectively). Moreover, the 
      percentage of diploid cells for chromosome 7 in renal cell carcinoma (RCC) with 
      venous involvement was significantly lower than those without venous involvement 
      (p = 0.023). These results suggest that the incidence of chromosomal aberrations 
      detected by FISH is more frequent than the chromosomal aneuploidy reported 
      previously by conventional cytogenetics. Therefore, loss of chromosome 3 may be 
      associated with an early event in RCC carcinogenesis. Gain of chromosomes 3 and 7 
      is correlated with tumor progression as well as gain and loss of chromosome 17. 
      Study of the chromosomal aberrations may provide a greater understanding of tumor 
      carcinogenesis and progression in RCC.
FAU - Wada, Y
AU  - Wada Y
AD  - Department of Urology, Shimane Medical University, Izumo, Japan.
FAU - Yokogi, H
AU  - Yokogi H
FAU - Moriyama-Gonda, N
AU  - Moriyama-Gonda N
FAU - Shigeno, K
AU  - Shigeno K
FAU - Shiina, H
AU  - Shiina H
FAU - Igawa, M
AU  - Igawa M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aneuploidy
MH  - *Chromosome Aberrations
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Kidney Neoplasms/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Trisomy
EDAT- 1997/11/14 00:00
MHDA- 1997/11/14 00:01
CRDT- 1997/11/14 00:00
PHST- 1997/11/14 00:00 [pubmed]
PHST- 1997/11/14 00:01 [medline]
PHST- 1997/11/14 00:00 [entrez]
AID - S0165460897002045 [pii]
AID - 10.1016/s0165-4608(97)00204-5 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1997 Nov;99(1):38-44. doi: 10.1016/s0165-4608(97)00204-5.