PMID- 9353321
OWN - NLM
STAT- MEDLINE
DCOM- 19971212
LR  - 20220408
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 272
IP  - 45
DP  - 1997 Nov 7
TI  - Tissue factor is induced by monocyte chemoattractant protein-1 in human aortic 
      smooth muscle and THP-1 cells.
PG  - 28568-73
AB  - Monocyte chemoattractant protein-1 (MCP-1) is a C-C chemokine thought to play a 
      major role in recruiting monocytes to the atherosclerotic plaque. Tissue factor 
      (TF), the initiator of coagulation, is found in the atherosclerotic plaque, 
      macrophages, and human aortic smooth muscle cells (SMC). The exposure of TF 
      during plaque rupture likely induces acute thrombosis, leading to myocardial 
      infarction and stroke. This report demonstrates that MCP-1 induces the 
      accumulation of TF mRNA and protein in SMC and in THP-1 myelomonocytic leukemia 
      cells. MCP-1 also induces TF activity on the surface of human SMC. The induction 
      of TF by MCP-1 in SMC is inhibited by pertussis toxin, suggesting that the SMC 
      MCP-1 receptor is coupled to a Gi-protein. Chelation of intracellular calcium and 
      inhibition of protein kinase C block the induction of TF by MCP-1, suggesting 
      that in SMC it is mediated by activation of phospholipase C. SMC bind MCP-1 with 
      a Kd similar to that previously reported for macrophages. However, mRNA encoding 
      the macrophage MCP-1 receptors, CCR2A and B, is not present in SMC, indicating 
      that they possess a distinct MCP-1 receptor. These data suggest that in addition 
      to being a chemoattractant, MCP-1 may have a procoagulant function and raise the 
      possibility of an autocrine pathway in which MCP-1, secreted by SMC and 
      macrophages, induces TF activity in these same cells.
FAU - Schecter, A D
AU  - Schecter AD
AD  - Cardiovascular Institute, The Mount Sinai School of Medicine, New York, New York 
      10029, USA.
FAU - Rollins, B J
AU  - Rollins BJ
FAU - Zhang, Y J
AU  - Zhang YJ
FAU - Charo, I F
AU  - Charo IF
FAU - Fallon, J T
AU  - Fallon JT
FAU - Rossikhina, M
AU  - Rossikhina M
FAU - Giesen, P L
AU  - Giesen PL
FAU - Nemerson, Y
AU  - Nemerson Y
FAU - Taubman, M B
AU  - Taubman MB
LA  - eng
GR  - HL 54469/HL/NHLBI NIH HHS/United States
GR  - HL09402/HL/NHLBI NIH HHS/United States
GR  - HL29019/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
RN  - 9035-58-9 (Thromboplastin)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Arteriosclerosis/metabolism
MH  - Calcium/metabolism
MH  - Cell Line
MH  - Chemokine CCL2/*pharmacology
MH  - Enzyme Activation
MH  - Humans
MH  - Monocytes/*drug effects/metabolism
MH  - Muscle, Smooth, Vascular/*drug effects/metabolism
MH  - Protein Kinase C/metabolism
MH  - RNA, Messenger/metabolism
MH  - Rabbits
MH  - Signal Transduction
MH  - Thromboplastin/genetics/*metabolism
MH  - Thrombosis/metabolism
EDAT- 1997/11/14 00:00
MHDA- 1997/11/14 00:01
CRDT- 1997/11/14 00:00
PHST- 1997/11/14 00:00 [pubmed]
PHST- 1997/11/14 00:01 [medline]
PHST- 1997/11/14 00:00 [entrez]
AID - S0021-9258(18)38693-9 [pii]
AID - 10.1074/jbc.272.45.28568 [doi]
PST - ppublish
SO  - J Biol Chem. 1997 Nov 7;272(45):28568-73. doi: 10.1074/jbc.272.45.28568.