PMID- 9355852
OWN - NLM
STAT- MEDLINE
DCOM- 19971120
LR  - 20061115
IS  - 0033-7587 (Print)
IS  - 0033-7587 (Linking)
VI  - 148
IP  - 5 Suppl
DP  - 1997 Nov
TI  - Biodosimetry results from space flight Mir-18.
PG  - S17-23
AB  - Astronauts are classified as radiation workers due to the presence of ionizing 
      radiation in space. For the assessment of health risks, physical dosimetry has 
      been indispensable. However, the change of the location of dosimeters on the crew 
      members, the variation in dose rate with location inside the spacecraft and the 
      unknown biological effects of microgravity can introduce significant 
      uncertainties in estimating exposure. To circumvent such uncertainty, a study on 
      the cytogenetic effects of space radiation in human lymphocytes was proposed and 
      conducted for Mir-18, a 115-day mission. This study used fluorescence in situ 
      hybridization (FISH) with whole-chromosome painting probes to score chromosomal 
      exchanges and the Giemsa staining method to determine the frequency of 
      dicentrics. The growth kinetics of cells and sister chromatid exchanges (SCEs) 
      were examined to ensure that chromosomal aberrations were scored in the first 
      mitosis and were induced primarily by space radiation. Our results showed that 
      the frequency of chromosomal aberrations increased significantly in postflight 
      samples compared to samples drawn prior to flight, and that the frequency of SCEs 
      was similar for both pre- and postflight samples. Based on a dose-response curve 
      for preflight samples exposed to gamma rays, the absorbed dose received by crew 
      members during the mission was estimated to be about 14.75 cSv. Because the 
      absorbed dose measured by physical dosimeters is 5.2 cGy for the entire mission, 
      the RBE is about 2.8.
FAU - Yang, T C
AU  - Yang TC
AD  - NASA Johnson Space Center, Houston, Texas 77058, USA.
FAU - George, K
AU  - George K
FAU - Johnson, A S
AU  - Johnson AS
FAU - Durante, M
AU  - Durante M
FAU - Fedorenko, B S
AU  - Fedorenko BS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Radiat Res
JT  - Radiation research
JID - 0401245
SB  - IM
MH  - *Astronauts
MH  - Chromosome Aberrations
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphocytes
MH  - *Radiation Dosage
MH  - Radiation Monitoring
MH  - Russia
MH  - Sister Chromatid Exchange
MH  - *Space Flight
OTO - NASA
OT  - NASA Center JSC
OT  - NASA Discipline Radiation Health
SFM - Flight Experiment
SFM - Mir Project
SFM - long duration
SFM - manned
FIR - Yang, T C
IR  - Yang TC
IRAD- JSC
EDAT- 1997/11/14 00:00
MHDA- 1997/11/14 00:01
CRDT- 1997/11/14 00:00
PHST- 1997/11/14 00:00 [pubmed]
PHST- 1997/11/14 00:01 [medline]
PHST- 1997/11/14 00:00 [entrez]
PST - ppublish
SO  - Radiat Res. 1997 Nov;148(5 Suppl):S17-23.