PMID- 9355862
OWN - NLM
STAT- MEDLINE
DCOM- 19971120
LR  - 20071114
IS  - 0033-7587 (Print)
IS  - 0033-7587 (Linking)
VI  - 148
IP  - 5 Suppl
DP  - 1997 Nov
TI  - Computer simulation of data on chromosome aberrations produced by X rays or alpha 
      particles and detected by fluorescence in situ hybridization.
PG  - S93-101
AB  - With fluorescence in situ hybridization (FISH), many different categories of 
      chromosome aberrations can be recognized-dicentrics, translocations, rings and 
      various complex aberrations such as insertions or three-way interchanges. 
      Relative frequencies for the various aberration categories indicate mechanisms of 
      radiation-induced damage and reflect radiation quality. Data obtained with FISH 
      support a proximity version of the classic random breakage-and-reunion model for 
      the formation of aberrations. A Monte Carlo computer implementation of the model, 
      called the CAS (chromosome aberration simulator), is generalized here to high 
      linear energy transfer (LET) and compared to published data for human cells 
      irradiated with X rays or 238Pu alpha particles. For each kind of radiation, the 
      CAS has two adjustable parameters: the number of interaction sites per cell 
      nucleus and the number of reactive double-strand breaks (DSBs) per gray. 
      Aberration frequencies for various painted chromosomes, of varying lengths, and 
      for 11 different categories of simple or complex aberrations were simulated and 
      compared to the data. The optimal number of interaction sites was found to be 
      approximately 13 for X irradiation and approximately 25 for alpha-particle 
      irradiation. The relative biological effectiveness (RBE) of alpha particles for 
      the induction of reactive DSBs (which are a minority of all DSBs) was found to be 
      approximately 4. The two-parameter CAS model adequately matches data for many 
      different categories of aberrations. It can use data obtained with FISH for any 
      one painting pattern to predict results for any other kind of painting pattern or 
      whole-genome staining, and to estimate a suggested overall numerical damage 
      indicator for chromosome aberration studies, the total misrejoining number.
FAU - Chen, A M
AU  - Chen AM
AD  - Department of Mathematics, University of California, Berkeley 94720, USA.
FAU - Lucas, J N
AU  - Lucas JN
FAU - Simpson, P J
AU  - Simpson PJ
FAU - Griffin, C S
AU  - Griffin CS
FAU - Savage, J R
AU  - Savage JR
FAU - Brenner, D J
AU  - Brenner DJ
FAU - Hlatky, L R
AU  - Hlatky LR
FAU - Sachs, R K
AU  - Sachs RK
LA  - eng
GR  - CA 63897-03/CA/NCI NIH HHS/United States
GR  - ES07361/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Radiat Res
JT  - Radiation research
JID - 0401245
SB  - IM
MH  - Alpha Particles
MH  - Cell Cycle
MH  - *Chromosome Aberrations
MH  - Chromosomes/*radiation effects
MH  - Computer Simulation
MH  - DNA Damage
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Linear Energy Transfer
MH  - Models, Biological
MH  - Radiometry/methods
MH  - X-Rays
OTO - NASA
OT  - Non-programmatic
EDAT- 1997/11/14 00:00
MHDA- 1997/11/14 00:01
CRDT- 1997/11/14 00:00
PHST- 1997/11/14 00:00 [pubmed]
PHST- 1997/11/14 00:01 [medline]
PHST- 1997/11/14 00:00 [entrez]
PST - ppublish
SO  - Radiat Res. 1997 Nov;148(5 Suppl):S93-101.