PMID- 9355863
OWN - NLM
STAT- MEDLINE
DCOM- 19971120
LR  - 20131121
IS  - 0033-7587 (Print)
IS  - 0033-7587 (Linking)
VI  - 148
IP  - 5 Suppl
DP  - 1997 Nov
TI  - Induction of chromosome aberrations in human cells by charged particles.
PG  - S102-7
AB  - Chromosome aberrations induced by high-energy charged particles in normal human 
      lymphocytes and human fibroblasts have been investigated. The charged particles 
      included 250 MeV/nucleon protons, 290 MeV/nucleon carbon ions and 1 GeV/nucleon 
      iron ions. The energies of the charged particles were higher than in most of the 
      studies reported in the literature. Lymphocytes were stimulated to grow 
      immediately after irradiation, while fibroblasts were incubated at 37 degrees C 
      for 24 h for repair. Chromosomes were collected at the first mitosis after 
      irradiation and chromosome aberrations were scored using the fluorescence in situ 
      hybridization (FISH) technique with a whole-chromosome 4 probe. Chromosome 
      aberrations were classified as reciprocal exchanges, incomplete exchanges, 
      deletions and complex exchanges. The relative biological effectiveness (RBE) for 
      each type of aberration was calculated by dividing a dose of 4 Gy by the dose of 
      the charged particles producing the same effect as 4 Gy of gamma rays. Results of 
      this study showed that complex aberrations have the highest RBE for radiation of 
      high linear energy transfer (LET) for human lymphocytes, but for fibroblasts, the 
      greatest effect was for incomplete exchanges. For both lymphocytes and 
      fibroblasts, iron ions induced a similar fraction of aberrant cells.
FAU - Wu, H
AU  - Wu H
AD  - NASA Johnson Space Center, Radiation Biology Laboratory, Houston, Texas 77058, 
      USA.
FAU - Durante, M
AU  - Durante M
FAU - George, K
AU  - George K
FAU - Yang, T C
AU  - Yang TC
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Radiat Res
JT  - Radiation research
JID - 0401245
RN  - 0 (Ions)
RN  - 0 (Protons)
RN  - 7440-44-0 (Carbon)
RN  - E1UOL152H7 (Iron)
SB  - IM
MH  - Carbon
MH  - Cells, Cultured
MH  - *Chromosome Aberrations
MH  - Dose-Response Relationship, Radiation
MH  - Fibroblasts/*radiation effects
MH  - Gamma Rays
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Ions
MH  - Iron
MH  - Lymphocytes/*radiation effects
MH  - Protons
OTO - NASA
OT  - NASA Center JSC
OT  - NASA Discipline Radiation Health
FIR - Yang, T C
IR  - Yang TC
IRAD- JSC
EDAT- 1997/11/14 00:00
MHDA- 1997/11/14 00:01
CRDT- 1997/11/14 00:00
PHST- 1997/11/14 00:00 [pubmed]
PHST- 1997/11/14 00:01 [medline]
PHST- 1997/11/14 00:00 [entrez]
PST - ppublish
SO  - Radiat Res. 1997 Nov;148(5 Suppl):S102-7.