PMID- 9359877
OWN - NLM
STAT- MEDLINE
DCOM- 19980205
LR  - 20220215
IS  - 0021-9533 (Print)
IS  - 0021-9533 (Linking)
VI  - 110 ( Pt 23)
DP  - 1997 Dec
TI  - HSF1 transcription factor concentrates in nuclear foci during heat shock: 
      relationship with transcription sites.
PG  - 2935-41
AB  - In this paper, we show that upon heat shock, HSF1 concentrates in the nucleus of 
      diploid human fibroblasts in two large foci. The relative distribution of HSF1 
      nuclear foci and active heat shock protein (hsp) genes was investigated by 
      combining fluorescence in situ hybridization (FISH) for the detection of hsp 
      nuclear transcripts and immunofluorescence for the detection of HSF1. We show 
      that the HSF1 foci are distinct from the sites of hsp70 and hsp90 genes 
      transcription. This is the second report of ploidy-dependent foci of 
      transcription factors that are independent of their specific transcription sites. 
      However, the correlation between the number of HSF1 foci and the ploidy of the 
      cells strongly supports the existence of a specific chromosomal target for HSF1 
      foci.
FAU - Jolly, C
AU  - Jolly C
AD  - DyOGen, INSERM U309, Institut Albert Bonniot, Domaine de la Merci, 38706, La 
      Tronche cedex, France. caroline.jolly@ujf-grenoble.fr
FAU - Morimoto, R
AU  - Morimoto R
FAU - Robert-Nicoud, M
AU  - Robert-Nicoud M
FAU - Vourc'h, C
AU  - Vourc'h C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Cell Sci
JT  - Journal of cell science
JID - 0052457
RN  - 0 (Chromatin)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (HSF1 protein, human)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (HSP90 Heat-Shock Proteins)
RN  - 0 (Heat Shock Transcription Factors)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Apoptosis
MH  - Cell Nucleus/*metabolism/ultrastructure
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Chromatin/physiology/ultrastructure
MH  - DNA-Binding Proteins/analysis/*biosynthesis
MH  - Diploidy
MH  - Female
MH  - Fibroblasts
MH  - HSP70 Heat-Shock Proteins/analysis/biosynthesis
MH  - HSP90 Heat-Shock Proteins/analysis/biosynthesis
MH  - Heat Shock Transcription Factors
MH  - Hot Temperature
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Kinetics
MH  - Skin/*cytology
MH  - Transcription Factors/biosynthesis
MH  - *Transcription, Genetic
EDAT- 1998/02/07 00:00
MHDA- 1998/02/07 00:01
CRDT- 1998/02/07 00:00
PHST- 1998/02/07 00:00 [pubmed]
PHST- 1998/02/07 00:01 [medline]
PHST- 1998/02/07 00:00 [entrez]
AID - 10.1242/jcs.110.23.2935 [doi]
PST - ppublish
SO  - J Cell Sci. 1997 Dec;110 ( Pt 23):2935-41. doi: 10.1242/jcs.110.23.2935.