PMID- 93610
OWN - NLM
STAT- MEDLINE
DCOM- 19800417
LR  - 20151119
IS  - 0022-1732 (Print)
IS  - 0022-1732 (Linking)
VI  - 23
IP  - 3
DP  - 1979
TI  - Comparison of auramine-rhodamine B and acridine orange for staining of acid-fast 
      bacteria.
PG  - 307-17
AB  - In cooperation of 6 laboratories in Czechoslovakia and in the GDR, the efficiency 
      of auramine-rhodamine B (AR) and acridine orange (AO) (short-time method) for 
      staining of acid-fast bacilli was compared. Whereas a former comparison of AR and 
      AO (original method) pointed out the superiority of AR, the investigation of both 
      methods used as short-time procedures showed significantly more acid-fast rods 
      after using AO. The number of "false positive" results was somewhat higher on AR 
      staining. However the results depend not only on the method used but also on the 
      procedure of staining and the optical equipment, and they are essentially 
      influenced by the experience and proficiency of the microscopist. Taking into 
      account the results of both studies both auramine-rhodamine B and acridine orange 
      can be proposed for the staining of slides for microscopical detection of 
      acid-fast rods. In case of AO, the short-time method is superior to the original 
      long-time procedure.
FAU - Kalich, R
AU  - Kalich R
FAU - Bennert, G
AU  - Bennert G
FAU - Fiedler, T
AU  - Fiedler T
FAU - Kubin, M
AU  - Kubin M
FAU - MezenskyL
AU  - MezenskyL
FAU - Turzova, M
AU  - Turzova M
FAU - Ulber, H
AU  - Ulber H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Czech Republic
TA  - J Hyg Epidemiol Microbiol Immunol
JT  - Journal of hygiene, epidemiology, microbiology, and immunology
JID - 2985116R
RN  - 0 (Aniline Compounds)
RN  - 0 (Rhodamines)
RN  - 0 (Xanthenes)
RN  - 2465-27-2 (Benzophenoneidum)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - *Acridine Orange
MH  - *Aniline Compounds
MH  - *Benzophenoneidum
MH  - False Positive Reactions
MH  - Microscopy, Fluorescence
MH  - Mycobacterium tuberculosis/*analysis
MH  - *Rhodamines
MH  - Staining and Labeling/methods
MH  - *Xanthenes
EDAT- 1979/01/01 00:00
MHDA- 1979/01/01 00:01
CRDT- 1979/01/01 00:00
PHST- 1979/01/01 00:00 [pubmed]
PHST- 1979/01/01 00:01 [medline]
PHST- 1979/01/01 00:00 [entrez]
PST - ppublish
SO  - J Hyg Epidemiol Microbiol Immunol. 1979;23(3):307-17.