PMID- 9361971
OWN - NLM
STAT- MEDLINE
DCOM- 19971204
LR  - 20190905
IS  - 0198-8859 (Print)
IS  - 0198-8859 (Linking)
VI  - 55
IP  - 2
DP  - 1997 Jul
TI  - New alleles of the HLA-B15 family.
PG  - 184-9
AB  - In a previous study of B locus alleles by sequence-specific oligonucleotide probe 
      (SSOP) hybridization, we observed 18 novel patterns in a panel of 360 
      individuals. Four of these novel patterns were caused by alleles of the human 
      leukocyte antigen (HLA)-B15 group, and three were available for this study. These 
      alleles were found in Oriental, Latin American, African American, and Caucasian 
      individuals. In addition, we analyzed a Caucasian subject who was found by 
      serology to have an unusual B15 specificity. We sequenced these four samples by 
      performing amplification from genomic DNA using polymerase chain reaction primers 
      designed to obtain HLA class I products that included exon 2 and exon 3 as well 
      as the intervening intron. The amplified segments were cloned and identified by 
      colony hybridization with nonradioactive SSOP. Nucleotide sequences were obtained 
      using an automated DNA sequencer. The allele B*1530 differs from B*1501 by a 
      substitution of Asp for Asn in position 114 and Ser for Tyr in codon 116. The new 
      allele B*1531 differs from B*1502 at amino acids 94, 95, and 152. The variant 
      B*1524 was found to have N-77, I-80, A-81, L-82, R-83. A similar motif exists in 
      B locus alleles that have the supertypic specificity Bw4 and in B*1513, B*1516, 
      B*1517, and B*1523; it is likely to have been generated by gene conversion. 
      Finally, the novel allele B*1527 is similar to B*1501 except for the presence of 
      Phe instead of Tyr at position 99. Because this change exists also in B*1506, it 
      is possible that B*1506 was derived from B*1501 through B*1527. It is of interest 
      that a similar substitution (Cys for Tyr at position 99) distinguishes A*0201 
      from A*0207 and is known to determine an epitope recognized by T cells. Thus, 
      B*1527 may also carry a change that is functionally relevant in cell-mediated 
      immunity.
FAU - Wang, J
AU  - Wang J
AD  - Department of Internal Medicine, University of Texas Southwestern Medical Center, 
      Dallas 75235-8886, USA.
FAU - Fernandez-Vina, M A
AU  - Fernandez-Vina MA
FAU - Lazaro, A M
AU  - Lazaro AM
FAU - Shumway, W
AU  - Shumway W
FAU - LeFor, W
AU  - LeFor W
FAU - Stastny, P
AU  - Stastny P
LA  - eng
GR  - 5 R01 HL47145/HL/NHLBI NIH HHS/United States
GR  - 5 U01 AI34621-03/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (HLA-B Antigens)
RN  - 0 (HLA-B15 Antigen)
SB  - IM
MH  - *Alleles
MH  - Base Sequence
MH  - HLA-B Antigens/*genetics
MH  - HLA-B15 Antigen
MH  - Humans
MH  - Molecular Sequence Data
EDAT- 1997/07/01 00:00
MHDA- 1997/11/15 00:01
CRDT- 1997/07/01 00:00
PHST- 1997/07/01 00:00 [pubmed]
PHST- 1997/11/15 00:01 [medline]
PHST- 1997/07/01 00:00 [entrez]
AID - S0198-8859(97)00090-6 [pii]
AID - 10.1016/s0198-8859(97)00090-6 [doi]
PST - ppublish
SO  - Hum Immunol. 1997 Jul;55(2):184-9. doi: 10.1016/s0198-8859(97)00090-6.