PMID- 9362357
OWN - NLM
STAT- MEDLINE
DCOM- 19971209
LR  - 20041117
IS  - 0270-9139 (Print)
IS  - 0270-9139 (Linking)
VI  - 26
IP  - 5
DP  - 1997 Nov
TI  - Fate of the human liver after hemihepatic portal vein embolization: cell kinetic 
      and morphometric study.
PG  - 1162-70
AB  - The favorable therapeutic effect of preoperative portal vein embolization (PVE) 
      was analyzed by assessing various volumetric, cell kinetic and morphometric 
      parameters and examining histologically the embolized and nonembolized lobes of 
      15 patients who underwent extended right lobectomy 2 to 3 weeks after PVE. Each 
      lobar volume was calculated from computed tomography (CT) images, hepatocyte 
      proliferation was evaluated by assessing proliferative cell nuclear antigen 
      (PCNA) expression and mitosis, hepatocyte apoptosis was evaluated by the terminal 
      deoxyribonucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay, 
      and the hepatocyte numerical density as well as the sinusoidal volumetric ratio 
      (Vvs) and the mean hepatocyte volume (Cv) were calculated using morphometric 
      methods. PVE induced hepatocyte apoptosis and atrophy of the embolized lobe (from 
      798 +/- 213 to 708 +/- 222 cm3; P < .05). The increased sinusoidal volume in this 
      lobe (17.7% +/- 4.5% and 21.7% +/- 5.7%, periportal and pericentral area, 
      respectively) may have been attributable to hepatocyte deletion. Cells in the 
      nonembolized lobe entered a highly active phase of proliferation within 2 weeks 
      after PVE. Further evidence of cellular proliferation was provided by the 
      increased nonembolized lobar volume (from 379 +/- 132 to 545 +/- 130 cm3; P < 
      .01) and numerical density of hepatocyte nuclei (Nv) (5.38 +/- 1.26 vs. 3.11 +/- 
      0.85 x 10(5)/mm3; P < .01, nonembolized vs. embolized lobe, respectively). In 
      conclusion, these results indicate that the favorable effect of PVE is 
      attributable to a net gain of functional hepatocyte mass and/or early induction 
      of hepatocyte proliferation following hepatectomy.
FAU - Harada, H
AU  - Harada H
AD  - First Department of Surgery, Faculty of Medicine, Shinshu University, School of 
      Medicine, Matsumoto, Japan.
FAU - Imamura, H
AU  - Imamura H
FAU - Miyagawa, S
AU  - Miyagawa S
FAU - Kawasaki, S
AU  - Kawasaki S
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Hepatology
JT  - Hepatology (Baltimore, Md.)
JID - 8302946
SB  - IM
MH  - Adult
MH  - Aged
MH  - Cell Cycle
MH  - *Embolization, Therapeutic
MH  - Female
MH  - Hepatectomy
MH  - Humans
MH  - Liver/*pathology
MH  - Liver Diseases/surgery
MH  - Male
MH  - Middle Aged
MH  - *Portal Vein
MH  - Preoperative Care
EDAT- 1997/11/15 00:00
MHDA- 1997/11/15 00:01
CRDT- 1997/11/15 00:00
PHST- 1997/11/15 00:00 [pubmed]
PHST- 1997/11/15 00:01 [medline]
PHST- 1997/11/15 00:00 [entrez]
AID - S0270-9139(97)00479-5 [pii]
AID - 10.1053/jhep.1997.v26.pm0009362357 [doi]
PST - ppublish
SO  - Hepatology. 1997 Nov;26(5):1162-70. doi: 10.1053/jhep.1997.v26.pm0009362357.