PMID- 9362374
OWN - NLM
STAT- MEDLINE
DCOM- 19971209
LR  - 20071114
IS  - 0270-9139 (Print)
IS  - 0270-9139 (Linking)
VI  - 26
IP  - 5
DP  - 1997 Nov
TI  - Mechanisms of intrathymic tolerance induction to isolated rat hepatocyte 
      allografts.
PG  - 1287-95
AB  - Intrathymic injection of alloantigen in young adult rats is capable of mediating 
      long-lived transplantation tolerance. In this study, we use a well-defined model 
      of isolated hepatocyte transplantation to define the mechanisms of intrathymic 
      induced tolerance. The recipient rats are Nagase analbuminemic rats (NAR) that 
      are deficient in albumin, to allow for following transplant acceptance using 
      metabolic and genetic markers. Tolerance to allogeneic hepatocyte transplants 
      could be mediated by intrathymic injection of live allogeneic splenocytes, 
      lethally irradiated splenocytes, or isolated hepatocytes. Intrathymic injection 
      of live allogeneic splenocytes, but not of hepatocytes or irradiated splenocytes, 
      resulted in donor microchimerism in peripheral lymphoid organs, with preferential 
      expansion of CD4-positive T cells in the recipient spleens. Tolerance could be 
      adoptively transferred from tolerant animals to naive recipients, but only from 
      those animals that had been inoculated with intrathymic donor splenocytes. We 
      conclude that donor microchimerism is found after intrathymic inoculation of live 
      splenocytes, but is not required for tolerance induction and that microchimerism 
      is not an absolute requirement for the generation of regulatory cells.
FAU - Attavar, P
AU  - Attavar P
AD  - Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461, 
      USA.
FAU - Budhai, L
AU  - Budhai L
FAU - Kim, B H
AU  - Kim BH
FAU - Roy-Chowdhury, N
AU  - Roy-Chowdhury N
FAU - Roy-Chowdhury, J
AU  - Roy-Chowdhury J
FAU - Davidson, A
AU  - Davidson A
LA  - eng
GR  - 5P30 DK41296 07/DK/NIDDK NIH HHS/United States
GR  - R01-DK 46057/DK/NIDDK NIH HHS/United States
GR  - R01-DK39137/DK/NIDDK NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Hepatology
JT  - Hepatology (Baltimore, Md.)
JID - 8302946
SB  - IM
MH  - Animals
MH  - Blotting, Southern
MH  - Cell Separation
MH  - *Cell Transplantation
MH  - Chimera/immunology
MH  - Flow Cytometry
MH  - Immune Tolerance/*physiology
MH  - Injections
MH  - Liver/*cytology
MH  - Polymerase Chain Reaction/methods
MH  - Rats
MH  - Rats, Inbred BN
MH  - Rats, Mutant Strains
MH  - Spleen/cytology
MH  - T-Lymphocytes/cytology
MH  - Thymus Gland/*immunology
MH  - Tissue Donors
EDAT- 1997/11/15 00:00
MHDA- 1997/11/15 00:01
CRDT- 1997/11/15 00:00
PHST- 1997/11/15 00:00 [pubmed]
PHST- 1997/11/15 00:01 [medline]
PHST- 1997/11/15 00:00 [entrez]
AID - S0270913997004965 [pii]
AID - 10.1002/hep.510260529 [doi]
PST - ppublish
SO  - Hepatology. 1997 Nov;26(5):1287-95. doi: 10.1002/hep.510260529.