PMID- 9363597
OWN - NLM
STAT- MEDLINE
DCOM- 19980127
LR  - 20190914
IS  - 0268-9146 (Print)
IS  - 0268-9146 (Linking)
VI  - 28
IP  - 5
DP  - 1997 Oct
TI  - Isolation, characterization and chromosomal localization of the porcine ciliary 
      neurotrophic factor (CNTF) gene.
PG  - 354-7
AB  - In the mouse, ciliary neurotrophic factor (CNTF) maintains embryonic stem cells 
      in an undifferentiated state; yet, the heterologous protein has no similar 
      effects on porcine embryonic stem (ES) cells. Consequently, we cloned and 
      sequenced the porcine CNTF gene and assigned it to chromosome 2. The CNTF gene 
      was found to contain two exons, which encoded a deduced polypeptide of 200 amino 
      acids in length with 83%, 82%, 82% and 81% amino acid similarity when compared to 
      known sequences in the rabbit, rat, human and mouse, respectively. Eight 
      non-conservative amino acid changes were identified in the porcine protein when 
      compared with other species. Comparison of the 5' region of the porcine CNTF and 
      other mammalian cytokines indicated the presence of several conserved 
      transcription-factor binding motifs, suggesting their importance for controlling 
      the specific expression of these proteins. In addition, CNTF was localized to 
      porcine chromosomes by fluorescence in situ hybridization (FISH). Chromosome arm 
      length ratios were calculated for 40 early metaphase chromosomes and 32 (80%) 
      indicated that the pig CNTF gene is located on chromosome 2p1.6. This was 
      confirmed by aligning R-banded FISH-labelled chromosomes to the standard porcine 
      ideogram.
FAU - Weaks, R L
AU  - Weaks RL
AD  - Department of Veterinary Anatomy and Public Health, College of Veterinary 
      Medicine, Texas A&M University, College Station 77843-4458, USA.
FAU - Ramsoondar, J J
AU  - Ramsoondar JJ
FAU - Gallagher, D S Jr
AU  - Gallagher DS Jr
FAU - Nogues, C
AU  - Nogues C
FAU - Piedrahita, J A
AU  - Piedrahita JA
LA  - eng
SI  - GENBANK/U57644
GR  - R01 HL551587/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Anim Genet
JT  - Animal genetics
JID - 8605704
RN  - 0 (Ciliary Neurotrophic Factor)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - *Chromosome Mapping
MH  - Ciliary Neurotrophic Factor
MH  - Conserved Sequence
MH  - Embryo, Mammalian
MH  - Exons
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Mice
MH  - Molecular Sequence Data
MH  - Nerve Growth Factors/*genetics
MH  - Nerve Tissue Proteins/biosynthesis/*genetics
MH  - Promoter Regions, Genetic
MH  - Rabbits
MH  - Rats
MH  - Sequence Alignment
MH  - Sequence Homology, Nucleic Acid
MH  - Stem Cells/cytology
MH  - Swine/*genetics
MH  - Transcription Factors/metabolism
EDAT- 1997/11/18 19:04
MHDA- 2001/03/28 10:01
CRDT- 1997/11/18 19:04
PHST- 1997/11/18 19:04 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1997/11/18 19:04 [entrez]
AID - 10.1111/j.1365-2052.1997.00159.x [doi]
PST - ppublish
SO  - Anim Genet. 1997 Oct;28(5):354-7. doi: 10.1111/j.1365-2052.1997.00159.x.