PMID- 9365831
OWN - NLM
STAT- MEDLINE
DCOM- 19980108
LR  - 20191024
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 20
IP  - 3
DP  - 1997 Nov
TI  - Relational mapping of MYCN and DDXI in band 2p24 and analysis of amplicon arrays 
      in double minute chromosomes and homogeneously staining regions by use of free 
      chromatin FISH.
PG  - 243-52
AB  - MYCN amplification has been observed in diverse neuronal tumors including 
      neuroblastoma, retinoblastoma, and small cell carcinoma of the lung, and has been 
      correlated with a poor prognosis in advanced-stage neuroblastomas. Recent studies 
      have shown a co-amplification of DDXI, a DEAD box gene, and MYCN in 
      retinoblastoma and neuroblastoma. DDXI has been mapped to within a megabase of 
      the MYCN gene in band 2p24. In the present study, the relational map of DDXI and 
      MYCN by fluorescence in situ hybridization (FISH) mapping to metaphase cells and 
      extended free chromatin fibers indicated that DDXI is telomeric to MYCN. 
      Dual-color FISH analysis of amplicons within arrays of extended chromatin fibers 
      was performed to examine the physical relationship of MYCN and DDXI within double 
      minute chromosomes (dmins) and homogeneously staining regions (hsrs). No regular 
      reiterated amplicon repeat unit was present in the hsrs, but detailed analysis of 
      the configurations of DDXI and MYCN within each array indicated that multiple 
      rearrangements generated a complex hsr amplicon structure. Similarly, analysis of 
      a cell line bearing dmins showed that a composite amplicon structure involving 
      deletions and/or duplications of MYCN and DDXI is a feature of dmin formation. 
      These data are consistent with a molecular mechanism involving many 
      rearrangements during the evolution of gene amplification, resulting in complex 
      amplicon structures with distinct changes in relative gene copy number and 
      considerable variation in intragenic distances between coamplified genes.
FAU - Pandita, A
AU  - Pandita A
AD  - Department of Medical Biophysics, University of Toronto, Ontario, Canada.
FAU - Godbout, R
AU  - Godbout R
FAU - Zielenska, M
AU  - Zielenska M
FAU - Thorner, P
AU  - Thorner P
FAU - Bayani, J
AU  - Bayani J
FAU - Squire, J A
AU  - Squire JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (Chromatin)
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Neoplasm)
RN  - EC 2.7.7.- (RNA Nucleotidyltransferases)
RN  - EC 3.6.1.- (DDX1 protein, human)
RN  - EC 3.6.4.13 (DEAD-box RNA Helicases)
RN  - EC 3.6.4.13 (RNA Helicases)
SB  - IM
MH  - Blotting, Southern
MH  - Chromatin
MH  - *Chromosome Mapping
MH  - Chromosomes, Human, Pair 2/*genetics
MH  - DEAD-box RNA Helicases
MH  - DNA Probes
MH  - DNA, Neoplasm
MH  - *Gene Amplification
MH  - *Gene Rearrangement
MH  - Genes, myc/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Multigene Family/genetics
MH  - *RNA Helicases
MH  - RNA Nucleotidyltransferases/*genetics
MH  - Tumor Cells, Cultured
EDAT- 1997/11/20 07:04
MHDA- 2000/06/20 09:00
CRDT- 1997/11/20 07:04
PHST- 1997/11/20 07:04 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1997/11/20 07:04 [entrez]
AID - 10.1002/(SICI)1098-2264(199711)20:3<243::AID-GCC4>3.0.CO;2-2 [pii]
AID - 10.1002/(sici)1098-2264(199711)20:3<243::aid-gcc4>3.0.co;2-2 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1997 Nov;20(3):243-52. doi: 
      10.1002/(sici)1098-2264(199711)20:3<243::aid-gcc4>3.0.co;2-2.