PMID- 9367162
OWN - NLM
STAT- MEDLINE
DCOM- 19971205
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1336
IP  - 3
DP  - 1997 Oct 20
TI  - Molecular cloning of the porcine beta-1,2-N-acetylglucosaminyltransferase II gene 
      and assignment to chromosome 1q23-q27.
PG  - 361-6
AB  - Glycosyltransferases play an important role in the synthesis of glycoproteins. 
      Here we report the isolation of a brain cDNA coding for 89% of the porcine 
      UDP-N-acetylglucosamine:alpha-6-D-mannoside-beta-1,2-N-acetylglucosaminy 
      ltransferase II (EC 2.4.1.143) (GnTII). The cDNA was used for screening a genomic 
      liver DNA library and isolation of a recombinant lambda FIX II phage containing 
      the complete porcine GnTII gene and upstream and downstream sequences. The 
      beta-1,2-N-acetylglucosaminyltransferase II gene harbours a single exon with an 
      open reading frame of 1338 bp coding for a 446 amino acid protein with a 
      calculated molecular mass of 51.1 kDa. The promoter of the GnTII gene is lacking 
      a TATA-box and shows variable transcription start sites. In the 3'-untranslated 
      region a polymorphic polyadenosine stretch was detected. The porcine GnTII gene 
      contains four polyadenylation sites. PCR analysis of a porcine-rodent hybrid cell 
      panel revealed the chromosomal location of the GnTII gene on SSC 1q23-q27. The 
      mapping data of the cell panel were confirmed by fluorescence in situ 
      hybridization (FISH) on metaphase chromosomes.
FAU - Leeb, T
AU  - Leeb T
AD  - Institute of Veterinary Medicine, University of Gottingen, Germany.
FAU - Kriegesmann, B
AU  - Kriegesmann B
FAU - Baumgartner, B G
AU  - Baumgartner BG
FAU - Klett, C
AU  - Klett C
FAU - Yerle, M
AU  - Yerle M
FAU - Hameister, H
AU  - Hameister H
FAU - Brenig, B
AU  - Brenig B
LA  - eng
SI  - GENBANK/Y09537
PT  - Journal Article
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (DNA Primers)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.4.1.- (N-Acetylglucosaminyltransferases)
RN  - EC 2.4.1.143 (alpha-1,6-mannosyl-glycoprotein 
      beta-1,2-N-acetylglucosaminyltransferase)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Brain/*enzymology
MH  - *Chromosome Mapping
MH  - Cloning, Organism
MH  - DNA Primers
MH  - Exons
MH  - Molecular Sequence Data
MH  - N-Acetylglucosaminyltransferases/*biosynthesis/chemistry/*genetics
MH  - Open Reading Frames
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Genetic
MH  - Recombinant Proteins/biosynthesis/chemistry
MH  - Regulatory Sequences, Nucleic Acid
MH  - Swine/*genetics
EDAT- 1997/11/21 00:00
MHDA- 1997/11/21 00:01
CRDT- 1997/11/21 00:00
PHST- 1997/11/21 00:00 [pubmed]
PHST- 1997/11/21 00:01 [medline]
PHST- 1997/11/21 00:00 [entrez]
AID - S0304-4165(97)00072-X [pii]
AID - 10.1016/s0304-4165(97)00072-x [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1997 Oct 20;1336(3):361-6. doi: 
      10.1016/s0304-4165(97)00072-x.