PMID- 9376663
OWN - NLM
STAT- MEDLINE
DCOM- 19971112
LR  - 20191102
IS  - 0950-3536 (Print)
IS  - 0950-3536 (Linking)
VI  - 10
IP  - 2
DP  - 1997 Jun
TI  - Cytogenetics of chronic myelogenous leukaemia.
PG  - 259-76
AB  - The Philadelphia (Ph) chromosome is present in the leukaemic cells of most 
      patients with chronic myelogenous leukaemia. Variant translocations occur in 10% 
      of patients but breakpoints on chromosomes 9 and 22 remain the same, so prognosis 
      of these patients is unchanged. Clonal evolution is infrequent in chronic phase 
      and its significance depends on the specific chromosome involved, the number of 
      metaphases affected and the timing in the chronic phase. The majority of patients 
      in blastic phase demonstrate clonal evolution; three specific abnormalities (+Ph, 
      +8 and isochromosome 17q) are present in 70% of patients. Loss of the Ph 
      chromosome on therapy is associated with prolonged survival. For monitoring these 
      events conventional G-band cytogenetics (CG) is essential at presentation to 
      characterize the Disease cytogenetically, while fluorescence in situ 
      hybridization (FISH) on hypermetaphase preparations (hypermetaphase FISH (HMF)) 
      is important for establishing the specific frequency of Ph+ cells. During 
      treatment FISH on interphase cells (I-FISH) can monitor the level of Ph+ cells in 
      circulation, while CG may be used to identify any suspected clonal evolution. 
      Where I-FISH is negative, HMF is essential to evaluate minimal residual disease.
FAU - O'Brien, S
AU  - O'Brien S
AD  - Department of Hematology, University of Texas M.D. Anderson Cancer, Center, 
      Houston 77030, USA.
FAU - Thall, P F
AU  - Thall PF
FAU - Siciliano, M J
AU  - Siciliano MJ
LA  - eng
GR  - CA16672/CA/NCI NIH HHS/United States
GR  - CA34936/CA/NCI NIH HHS/United States
GR  - CA49639/CA/NCI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PT  - Review
PL  - England
TA  - Baillieres Clin Haematol
JT  - Bailliere's clinical haematology
JID - 8800474
SB  - IM
MH  - *Chromosomes, Human, Pair 12
MH  - *Chromosomes, Human, Pair 9
MH  - Cytogenetics
MH  - Humans
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*genetics
MH  - *Philadelphia Chromosome
RF  - 83
EDAT- 1997/06/01 00:00
MHDA- 1997/10/23 00:01
CRDT- 1997/06/01 00:00
PHST- 1997/06/01 00:00 [pubmed]
PHST- 1997/10/23 00:01 [medline]
PHST- 1997/06/01 00:00 [entrez]
AID - 10.1016/s0950-3536(97)80006-6 [doi]
PST - ppublish
SO  - Baillieres Clin Haematol. 1997 Jun;10(2):259-76. doi: 
      10.1016/s0950-3536(97)80006-6.