PMID- 9378507
OWN - NLM
STAT- MEDLINE
DCOM- 19971107
LR  - 20190512
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 91
IP  - 4
DP  - 1997 Aug
TI  - Characterization of a constitutive type III nitric oxide synthase in human U937 
      monocytic cells: stimulation by soluble CD23.
PG  - 643-8
AB  - The soluble cleavage fragment of the low-affinity immunoglobulin E (IgE) 
      receptor/CD23 (sCD23 25000 MW) and antibodies directed against their receptors on 
      monocytes, CD11b and CD11c, stimulate the production of nitric oxide (NO) by 
      these cells and we have suggested that the enzyme involved could be related to 
      the endothelial constitutive type III nitric oxide synthase (ecNOS). In the 
      present work, we have analysed the characteristic properties of this NOS isoform 
      in the model of the human promonocytic cells U937 By reverse-transcription 
      polymerase chain reaction (RT-PCR), the presence of an mRNA coding for type III 
      NOS was found in U937 cells and the corresponding protein was detected by 
      immunofluorescence in permeabilized cells with a specific anti-ecNOS monoclonal 
      antibody (mAb). Membrane extracts displayed a NOS activity dependent on the 
      presence of calcium and calmodulin in the reaction medium and that was abrogated 
      in the presence of EGTA. Recombinant soluble CD23 (25000 MW) was found to trigger 
      an NO-dependent cGMP accumulation in these cells, which was abrogated by calcium 
      chelators and inhibitors of the calcium/calmodulin complex. Moreover, sCD23 
      elicited a transient augmentation of intracytoplasmic free calcium concentration 
      [Ca2+]i that was dependent on the presence of calcium in the external buffer and 
      was prevented in the presence of EGTA, indicating that it was due to a calcium 
      influx. In conclusion, human promonocytic cells such as U937 exhibit a functional 
      type III NOS that can be stimulated by calcium-raising agents, such as sCD23.
FAU - Roman, V
AU  - Roman V
AD  - INSERM U365, Institut Curie, Paris, France.
FAU - Dugas, N
AU  - Dugas N
FAU - Abadie, A
AU  - Abadie A
FAU - Amirand, C
AU  - Amirand C
FAU - Zhao, H
AU  - Zhao H
FAU - Dugas, B
AU  - Dugas B
FAU - Kolb, J P
AU  - Kolb JP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, IgE)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - H2D2X058MU (Cyclic GMP)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Calcium/metabolism
MH  - Cyclic GMP/metabolism
MH  - Gene Expression
MH  - Humans
MH  - Monocytes/*enzymology/*immunology/metabolism
MH  - Nitric Oxide Synthase/genetics/*metabolism
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/genetics
MH  - Receptors, IgE/*immunology
MH  - Solubility
MH  - Tumor Cells, Cultured
PMC - PMC1363888
EDAT- 1997/08/01 00:00
MHDA- 1997/10/24 00:01
PMCR- 1998/08/01
CRDT- 1997/08/01 00:00
PHST- 1997/08/01 00:00 [pubmed]
PHST- 1997/10/24 00:01 [medline]
PHST- 1997/08/01 00:00 [entrez]
PHST- 1998/08/01 00:00 [pmc-release]
AID - 10.1046/j.1365-2567.1997.d01-2263.x [doi]
PST - ppublish
SO  - Immunology. 1997 Aug;91(4):643-8. doi: 10.1046/j.1365-2567.1997.d01-2263.x.