PMID- 9379920
OWN - NLM
STAT- MEDLINE
DCOM- 19971110
LR  - 20190512
IS  - 0267-8357 (Print)
IS  - 0267-8357 (Linking)
VI  - 12
IP  - 5
DP  - 1997 Sep
TI  - Characterization of SV40-transformed xeroderma pigmentosum cell lines for their 
      usability in HPRT mutation studies.
PG  - 391-5
AB  - We have characterized six SV40-transformed xeroderma pigmentosum cell lines 
      (XP20S and XP12BE derived from female donors, XP12RO-SV, XP3BR/12SV, XP4PA-SV and 
      XP8CAC-SV from male donors) for their usability in HPRT mutation studies. All 
      cell lines exhibit hypersensitivity, compared with MRC5CV1 cells, towards the 
      cytotoxic action of UV-irradiation. They were all shown to be heteronuclear and 
      hyperdiploid with pronounced variability in chromosome number. Fluorescence in 
      situ hybridization (FISH) with an X-chromosomal library (X-chromosome painting) 
      and BrdUrd-labelling of late-replicating X-chromosomes demonstrated the presence 
      of variable numbers of X-chromosomes and additional X-chromosomal material and 
      suggested the presence of more than one genetically active HPRT allele in the 
      majority of cells of five cell lines. The cell line XP8CAC-SV (complementation 
      group C) seemed to be most suitable for HPRT mutation studies due to its 
      near-diploid karyotype with only one X-chromosome in the majority of cells. From 
      this cell line, a clonal subline was established (XP8CAC-SV-C1) which revealed 
      the same UV-hypersensitivity as the parental cell line and a near-diploid 
      karyotype with one X-chromosome in 94% of the metaphases. Molecular analysis of 
      the HPRT gene gave a normal PCR amplification pattern for all exons and the 
      normal wild-type sequence of the cDNA. HPRT tests with 
      (+)-anti-benzo[a]pyrene-7,8-diol-9,10-oxide [(+)-anti-BPDE] showed a 
      reproducible, concentration related increase in mutant frequencies. Compared with 
      results with MRC5CV1 cells, the obtained data indicate spontaneous and 
      (+)-anti-BPDE-induced hypermutability of the XP line. XP8CAC-SV-C1 thus 
      represents a permanent XP cell line with characteristic cellular XP features 
      which is convenient for studying the influence of deficient excision repair on 
      HPRT mutant frequencies and mutation spectra.
FAU - Merk, O
AU  - Merk O
AD  - Universitat Ulm, Abteilung Medizinische Genetik, Germany.
FAU - Speit, G
AU  - Speit G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mutagenesis
JT  - Mutagenesis
JID - 8707812
RN  - 55097-80-8 (7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide)
RN  - EC 2.4.2.8 (Hypoxanthine Phosphoribosyltransferase)
SB  - IM
MH  - 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide/toxicity
MH  - Cell Line
MH  - Cell Line, Transformed
MH  - Cell Survival/radiation effects
MH  - *Cell Transformation, Viral
MH  - Female
MH  - Fibroblasts
MH  - Genetic Complementation Test
MH  - Humans
MH  - Hypoxanthine Phosphoribosyltransferase/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mutagenesis
MH  - Mutagenicity Tests/*methods
MH  - Polymerase Chain Reaction
MH  - Sex Chromosome Aberrations
MH  - Simian virus 40/*genetics
MH  - Skin/cytology/radiation effects
MH  - Translocation, Genetic
MH  - *Ultraviolet Rays
MH  - X Chromosome/*radiation effects
MH  - Xeroderma Pigmentosum
EDAT- 1997/10/06 00:00
MHDA- 1997/10/06 00:01
CRDT- 1997/10/06 00:00
PHST- 1997/10/06 00:00 [pubmed]
PHST- 1997/10/06 00:01 [medline]
PHST- 1997/10/06 00:00 [entrez]
AID - 10.1093/mutage/12.5.391 [doi]
PST - ppublish
SO  - Mutagenesis. 1997 Sep;12(5):391-5. doi: 10.1093/mutage/12.5.391.