PMID- 9380277
OWN - NLM
STAT- MEDLINE
DCOM- 19971107
LR  - 20180214
IS  - 0028-3835 (Print)
IS  - 0028-3835 (Linking)
VI  - 66
IP  - 3
DP  - 1997 Sep
TI  - Responses of melanin-concentrating hormone mRNA to salt water challenge in the 
      rainbow trout.
PG  - 195-202
AB  - Melanin-concentrating hormone (MCH) is a structurally conserved neuropeptide, 
      produced in the hypothalamus of all vertebrates where it probably serves as a 
      central neurotransmitter/neuromodulator. In teleost fish it is also a 
      neurohypophysial hormone with peripheral effects on skin colour but its central 
      effects are less well understood. In mammals, MCH mRNA abundance changes in 
      response to salt-loading or dehydration, suggesting an involvement in salt or 
      water balance. The present study has used in situ hybridization to investigate 
      the response of the MCH neurons in the rainbow trout (Oncorhynchus mykiss) to 
      progressive changes in ambient salinity. In trout, MCH perikarya are found in two 
      hypothalamic sites: predominantly in the nucleus lateralis tuberis (NLT) and, to 
      a lesser extent, in neurons above the lateral ventricular recess (LVR). Immersion 
      in 50% salt water (SW) for 24 h had no effects on MCH transcripts, plasma osmotic 
      pressure (OP) or cortisol concentrations, but after 24 h in 80% SW, plasma OP and 
      cortisol were raised and MCH transcripts in the NLT were significantly increased 
      (159% of controls, p < 0.01). LVR-MCH neurons remained unaffected. However, after 
      24 h in 100% SW, MCH mRNA was significantly reduced in both groups of neurons 
      (NLT -62% of controls, p < 0.001; LVR -33% of control, p < 0.001). These 
      responses were transient and were no longer apparent after 6 days in 100% SW, 
      despite the fact that plasma OP and cortisol levels continued to rise. The 
      relative importance of osmotic disturbance and stress on the differential 
      responses of the 2 groups of MCH neurons to changing salinity is discussed, 
      together with a consideration of the potential role of MCH in osmoregulation.
FAU - Francis, K
AU  - Francis K
AD  - School of Biology and Biochemistry, University of Bath, UK.
FAU - Suzuki, M
AU  - Suzuki M
FAU - Baker, B I
AU  - Baker BI
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Neuroendocrinology
JT  - Neuroendocrinology
JID - 0035665
RN  - 0 (Hypothalamic Hormones)
RN  - 0 (Melanins)
RN  - 0 (Pituitary Hormones)
RN  - 0 (RNA, Messenger)
RN  - 67382-96-1 (melanin-concentrating hormone)
RN  - WI4X0X7BPJ (Hydrocortisone)
SB  - IM
MH  - Animals
MH  - Hydrocortisone/blood
MH  - Hypothalamic Hormones/*genetics
MH  - In Situ Hybridization
MH  - Melanins/*genetics
MH  - *Melanophores
MH  - Oncorhynchus mykiss/*metabolism
MH  - Osmotic Pressure
MH  - Pituitary Hormones/*genetics
MH  - RNA, Messenger/*biosynthesis
MH  - Seawater/*adverse effects
EDAT- 1997/10/06 00:00
MHDA- 1997/10/06 00:01
CRDT- 1997/10/06 00:00
PHST- 1997/10/06 00:00 [pubmed]
PHST- 1997/10/06 00:01 [medline]
PHST- 1997/10/06 00:00 [entrez]
AID - 10.1159/000127238 [doi]
PST - ppublish
SO  - Neuroendocrinology. 1997 Sep;66(3):195-202. doi: 10.1159/000127238.