PMID- 9382887
OWN - NLM
STAT- MEDLINE
DCOM- 19971230
LR  - 20240214
IS  - 0022-1007 (Print)
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Linking)
VI  - 186
IP  - 11
DP  - 1997 Dec 1
TI  - A novel antioxidant gene from Mycobacterium tuberculosis.
PG  - 1885-96
AB  - Among the major antimicrobial products of macrophages are reactive intermediates 
      of the oxidation of nitrogen (RNI) and the reduction of oxygen (ROI). Selection 
      of recombinants in acidified nitrite led to the cloning of a novel gene, noxR1, 
      from a pathogenic clinical isolate of Mycobacterium tuberculosis. Expression of 
      noxR1 conferred upon Escherichia coli and Mycobacterium smegmatis enhanced 
      ability to resist RNI and ROI, whether the bacteria were exposed to exogenous 
      compounds in medium or to endogenous products in macrophages. These studies 
      provide the first identification of an RNI resistance mechanism in mycobacteria, 
      point to a new mechanism for resistance to ROI, and raise the possibility that 
      inhibition of the noxR1 pathway might enhance the ability of macrophages to 
      control tuberculosis.
FAU - Ehrt, S
AU  - Ehrt S
AD  - Division of International Medicine and Infectious Disease, Department of 
      Medicine, Cornell University Medical College, New York 10021, USA.
FAU - Shiloh, M U
AU  - Shiloh MU
FAU - Ruan, J
AU  - Ruan J
FAU - Choi, M
AU  - Choi M
FAU - Gunzburg, S
AU  - Gunzburg S
FAU - Nathan, C
AU  - Nathan C
FAU - Xie, Q
AU  - Xie Q
FAU - Riley, L W
AU  - Riley LW
LA  - eng
SI  - GENBANK/Y08323
GR  - T32 GM007739/GM/NIGMS NIH HHS/United States
GR  - GM-07739/GM/NIGMS NIH HHS/United States
GR  - HL-51967/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Antioxidants)
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Nitrites)
RN  - 0 (NoxR1 protein, Mycobacterium tuberculosis)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Recombinant Fusion Proteins)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
EIN - J Exp Med 1998 Jan 5;187(1):141
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antioxidants
MH  - Bacterial Proteins/*genetics/physiology
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA, Bacterial/genetics
MH  - Escherichia coli/genetics
MH  - *Genes, Bacterial
MH  - Hydrogen Peroxide/metabolism
MH  - Macrophages/metabolism/microbiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - Mycobacterium/genetics
MH  - Mycobacterium tuberculosis/*genetics
MH  - Nitrites/*metabolism
MH  - Oxidation-Reduction
MH  - Reactive Oxygen Species/*metabolism
MH  - Recombinant Fusion Proteins/metabolism
MH  - Transfection
MH  - Tuberculosis/immunology
PMC - PMC2199150
EDAT- 1998/01/07 00:00
MHDA- 1998/01/07 00:01
PMCR- 1998/06/01
CRDT- 1998/01/07 00:00
PHST- 1998/01/07 00:00 [pubmed]
PHST- 1998/01/07 00:01 [medline]
PHST- 1998/01/07 00:00 [entrez]
PHST- 1998/06/01 00:00 [pmc-release]
AID - 10.1084/jem.186.11.1885 [doi]
PST - ppublish
SO  - J Exp Med. 1997 Dec 1;186(11):1885-96. doi: 10.1084/jem.186.11.1885.