PMID- 9384608
OWN - NLM
STAT- MEDLINE
DCOM- 19980226
LR  - 20190512
IS  - 0964-6906 (Print)
IS  - 0964-6906 (Linking)
VI  - 7
IP  - 1
DP  - 1998 Jan
TI  - The mouse H19 locus mediates a transition between imprinted and non-imprinted DNA 
      replication patterns.
PG  - 91-5
AB  - Genes subject to genomic imprinting generally occur in clusters of hundreds of 
      kilobases. These domains exhibit several gamete of origin-dependent 
      manifestations, including a pattern of asynchronous replication when studied by 
      fluorescence in situ hybridization (FISH). We find a transition from asynchronous 
      replication at the imprinted mouse H19 gene to synchronous replication at the 
      downstream Rpl23 gene, the human homologue of which appears to be non-imprinted. 
      Two-colour FISH demonstrates that this transition is due solely to a difference 
      in replication timing between the upstream and downstream chromatin on the 
      later-replicating (maternal) chromosome. This difference is lost in mice deleted 
      for the H19 gene body and 9.9 kb of upstream DNA when this deletion is maternally 
      inherited, with synchronous replication patterns extending over 110 kb upstream 
      from the deleted area. No effect is seen when the deletion is paternally 
      inherited. The presence of a boundary element in this region has been suggested 
      by observations of position-independent expression of H19 -containing transgenes 
      and the blocking of accessibility of downstream enhancers to the upstream Igf2 
      and Ins2 genes on the maternal chromosome. The FISH studies presented here 
      demonstrate the insulation of replication patterns within the imprinted domain 
      from downstream, non-imprinted chromatin, mediated by an element at the H19 locus 
      which is subject to genomic imprinting.
FAU - Greally, J M
AU  - Greally JM
AD  - Department of Genetics, Yale University School of Medicine, 333 Cedar Street, PO 
      Box 208081, New Haven, CT 06520-8081, USA.
FAU - Starr, D J
AU  - Starr DJ
FAU - Hwang, S
AU  - Hwang S
FAU - Song, L
AU  - Song L
FAU - Jaarola, M
AU  - Jaarola M
FAU - Zemel, S
AU  - Zemel S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Hum Mol Genet
JT  - Human molecular genetics
JID - 9208958
RN  - 0 (H19 long non-coding RNA)
RN  - 0 (Muscle Proteins)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Untranslated)
SB  - IM
MH  - Animals
MH  - Chromosome Mapping
MH  - DNA Replication/*genetics
MH  - Genome
MH  - Humans
MH  - Mice
MH  - Muscle Proteins/*genetics
MH  - RNA, Long Noncoding
MH  - *RNA, Untranslated
EDAT- 1998/02/28 00:00
MHDA- 1998/02/28 00:01
CRDT- 1998/02/28 00:00
PHST- 1998/02/28 00:00 [pubmed]
PHST- 1998/02/28 00:01 [medline]
PHST- 1998/02/28 00:00 [entrez]
AID - ddb018 [pii]
AID - 10.1093/hmg/7.1.91 [doi]
PST - ppublish
SO  - Hum Mol Genet. 1998 Jan;7(1):91-5. doi: 10.1093/hmg/7.1.91.