PMID- 9385371
OWN - NLM
STAT- MEDLINE
DCOM- 19971224
LR  - 20190722
IS  - 0340-6717 (Print)
IS  - 0340-6717 (Linking)
VI  - 101
IP  - 1
DP  - 1997 Nov
TI  - Alphoid variant-specific FISH probes can distinguish autosomal meiosis I from 
      meiosis II non-disjunction in human sperm.
PG  - 61-6
AB  - Over the past few years, several groups have used fluorescence in situ 
      hybridization (FISH) to study aneuploidy in human sperm. Several important 
      observations have derived from these studies, including the demonstration of 
      chromosome-specific variation in non-disjunction frequencies, and the possible 
      association of aneuploidy with environmental agents and with increasing paternal 
      age. However, an important technical limitation of these studies has been the 
      inability to distinguish between autosomal non-disjunction occurring at meiosis I 
      and meiosis II. In the present report, we describe a simple FISH-based approach 
      designed to overcome this limitation. Using oligonucleotide probes capable of 
      distinguishing subtle differences in the alpha satellite sequences of chromosome 
      17, we demonstrate that (in appropriate heterozygotes) it is possible to 
      simultaneously identify disomic sperm and to determine the meiotic stage of 
      origin of the additional chromosome. This novel approach has important 
      implications for future FISH sperm studies, since the ability to distinguish 
      between meiosis I and meiosis II non-disjunction will make it possible to 
      determine whether putative etiological agents affect chromosome segregation at 
      both, or only one, of the two meiotic stages.
FAU - O'Keefe, C L
AU  - O'Keefe CL
AD  - Department of Genetics, Case Western Reserve University, Cleveland, Ohio, USA.
FAU - Griffin, D K
AU  - Griffin DK
FAU - Bean, C J
AU  - Bean CJ
FAU - Matera, A G
AU  - Matera AG
FAU - Hassold, T J
AU  - Hassold TJ
LA  - eng
GR  - HD21341/HD/NICHD NIH HHS/United States
GR  - HD32111/HD/NICHD NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Germany
TA  - Hum Genet
JT  - Human genetics
JID - 7613873
RN  - 0 (DNA, Satellite)
RN  - 0 (Oligonucleotide Probes)
SB  - IM
MH  - Base Sequence
MH  - Chromosomes, Human, Pair 17/genetics
MH  - DNA, Satellite/genetics
MH  - Heterozygote
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Meiosis/*genetics
MH  - *Nondisjunction, Genetic
MH  - Oligonucleotide Probes
MH  - Spermatozoa/*ultrastructure
EDAT- 1998/01/07 00:00
MHDA- 1998/01/07 00:01
CRDT- 1998/01/07 00:00
PHST- 1998/01/07 00:00 [pubmed]
PHST- 1998/01/07 00:01 [medline]
PHST- 1998/01/07 00:00 [entrez]
AID - 10.1007/s004390050587 [doi]
PST - ppublish
SO  - Hum Genet. 1997 Nov;101(1):61-6. doi: 10.1007/s004390050587.