PMID- 9387708
OWN - NLM
STAT- MEDLINE
DCOM- 19971216
LR  - 20061115
IS  - 0529-5815 (Print)
IS  - 0529-5815 (Linking)
VI  - 34
IP  - 5
DP  - 1996 May
TI  - [Different proteins in NRCF (nerve regeneration conditioned fluid) from the 
      proximal and distal nerve stump].
PG  - 297-9
AB  - 80 SD rats were randomly divided into four groups of 20 each; electrostimulation 
      of myeloneure (Group N); electrostimulation of the denervated muscle (Group M); 
      Group C1; and Goup C2. The left lateral sciatic nerve of rats was exercised 2mm 
      in length, and the distal nerve stump of the goup C2 and M and proximal nerve 
      stamp of the Group C1 and N were each sulured into the silcon chamber with the 
      opposile end ligated so that a 10mm was left between the stump and the blind end 
      of chamber. At 3rd, 7th, 14th, 21th and 28th day after nerve transection and tube 
      implantation, the fluid in the silicon tube was aspirated. The samples of fluid 
      were spun and 4 microliters aliguots were taken from the super ratant. Then 
      subjected to the aliquots were taken by phase system and assayed by Gel scan 
      system. The results showed that the Group C2 and M had highly increase in the 
      protein amount. Than that of group C1 and N in the range of 6-10 KD molecular 
      weight. In 29-47 KD molecular weight range, the variety of protein molecular 
      weight in aliguots of the Group N had and obvious increase compared with the 
      group C1. In 16-29 KD, the protein amount in the Group M was more than that in 
      the Group C2.
FAU - Li, Q
AU  - Li Q
AD  - Department of Hand Surgery, Huashan Hospital, Shanghai.
FAU - Gu, Y
AU  - Gu Y
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Wai Ke Za Zhi
JT  - Zhonghua wai ke za zhi [Chinese journal of surgery]
JID - 0153611
RN  - 0 (Nerve Tissue Proteins)
SB  - IM
MH  - Animals
MH  - Electric Stimulation
MH  - *Nerve Regeneration
MH  - Nerve Tissue Proteins/*metabolism
MH  - Neurons/physiology
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sciatic Nerve/injuries/*physiology
EDAT- 1996/05/01 00:00
MHDA- 1997/12/05 00:01
CRDT- 1996/05/01 00:00
PHST- 1996/05/01 00:00 [pubmed]
PHST- 1997/12/05 00:01 [medline]
PHST- 1996/05/01 00:00 [entrez]
PST - ppublish
SO  - Zhonghua Wai Ke Za Zhi. 1996 May;34(5):297-9.