PMID- 9387929
OWN - NLM
STAT- MEDLINE
DCOM- 19980206
LR  - 20191102
IS  - 0948-6143 (Print)
IS  - 0948-6143 (Linking)
VI  - 108
IP  - 4-5
DP  - 1997 Oct-Nov
TI  - The U22 host gene (UHG): chromosomal localization of UHG and distribution of U22 
      small nucleolar RNA.
PG  - 365-70
AB  - The U22 host gene (UHG) is very unusual because it encodes a spliced, 
      polyadenylated RNA that has little apparent coding capacity and is rapidly 
      degraded. The stable RNA products from this locus are actually encoded within 
      eight different introns of the UHG pre-RNA. These small nucleolar RNAs (snoRNAs) 
      assemble into ribonucleoproteins, some of which have been shown to function in 
      rRNA processing and modification. In order to more fully characterize the locus, 
      we have mapped UHG to chromosome 11q13 by fluorescence in situ hybridization 
      (FISH). Radiation hybrid mapping placed this sequence-tagged site with very high 
      probability (lod >19) to chromosome 11, approximately 10.1 cR distal to framework 
      marker WI-8652. We also investigated the possibility that the expression of UHG 
      was subject to genomic imprinting. Several laboratories have shown that 
      non-protein-coding mRNAs are frequently associated with imprinted domains in 
      mammalian cells. We used a novel somatic cell hybrid method to assay 
      parent-of-origin effects in the expression of UHG alleles and found that, unlike 
      XIST, IPW, and H19, this RNA is expressed biparentally. Additional FISH 
      experiments using anti-U22 oligonucleotides revealed that, as with U3, this 
      sno-RNA is localized throughout the nucleolus.
FAU - Frey, M R
AU  - Frey MR
AD  - Department of Genetics, Case Western Reserve University, Cleveland, OH 
      44106-4955, USA.
FAU - Wu, W
AU  - Wu W
FAU - Dunn, J M
AU  - Dunn JM
FAU - Matera, A G
AU  - Matera AG
LA  - eng
SI  - GENBANK/AF081279
GR  - GM-53034/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Germany
TA  - Histochem Cell Biol
JT  - Histochemistry and cell biology
JID - 9506663
RN  - 0 (RNA, Small Nuclear)
SB  - IM
MH  - Cell Nucleolus/metabolism
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 11
MH  - Humans
MH  - Hybrid Cells
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - RNA, Small Nuclear/*analysis
EDAT- 1997/12/05 00:00
MHDA- 1997/12/05 00:01
CRDT- 1997/12/05 00:00
PHST- 1997/12/05 00:00 [pubmed]
PHST- 1997/12/05 00:01 [medline]
PHST- 1997/12/05 00:00 [entrez]
AID - 10.1007/s004180050177 [doi]
PST - ppublish
SO  - Histochem Cell Biol. 1997 Oct-Nov;108(4-5):365-70. doi: 10.1007/s004180050177.