PMID- 9388069
OWN - NLM
STAT- MEDLINE
DCOM- 19971208
LR  - 20061115
IS  - 0893-3952 (Print)
IS  - 0893-3952 (Linking)
VI  - 10
IP  - 11
DP  - 1997 Nov
TI  - Applications of single-color and double-color oligonucleotide primed in situ 
      labeling in cytology.
PG  - 1164-71
AB  - Interphase cytogenetics is mostly performed with use of fluorescence in situ 
      hybridization (FISH), using long DNA probes of several hundred or thousand base 
      pairs in length. Recently, oligonucleotide primed in situ labeling (PRINS) was 
      established for staining centromeres and telomeres of chromosomes in metaphase 
      spreads by Taq-polymerase-mediated incorporation of labeled nucleotides. We 
      investigated the use of PRINS in intact interphase cells of various cytologic 
      preparations, targeting chromosomes 1, 8, and 9. Examining cell smears (n = 3), 
      touch preparations (n = 20), and cytospins (n = 11) of non-neoplastic and 
      neoplastic tissues, PRINS was as sensitive and reliable as the FISH method in 
      assessing the exact chromosome number. Aneuploidy in tumor cells was confirmed by 
      double-color PRINS in a part of the specimens. The PRINS reaction, which requires 
      heating of the cell preparations to as high as 96 degrees C, did not affect the 
      cytomorphologic details. Because PRINS is much faster and approximately 10 times 
      less expensive than FISH, this method allows an increased application of 
      interphase cytogenetics in diagnostic cytopathology.
FAU - Werner, M
AU  - Werner M
AD  - Institute of Pathology, Technical University, Munich, Germany.
FAU - Nasarek, A
AU  - Nasarek A
FAU - Tchinda, J
AU  - Tchinda J
FAU - von Wasielewski, R
AU  - von Wasielewski R
FAU - Komminoth, P
AU  - Komminoth P
FAU - Wilkens, L
AU  - Wilkens L
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Mod Pathol
JT  - Modern pathology : an official journal of the United States and Canadian Academy 
      of Pathology, Inc
JID - 8806605
RN  - 0 (Oligonucleotide Probes)
SB  - IM
MH  - *Aneuploidy
MH  - Chromosomes, Human, Pair 1/*genetics
MH  - Chromosomes, Human, Pair 8/*genetics
MH  - Chromosomes, Human, Pair 9/*genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization/*methods
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Male
MH  - Neoplasms/*genetics/pathology
MH  - Oligonucleotide Probes/genetics
EDAT- 1997/12/05 00:00
MHDA- 1997/12/05 00:01
CRDT- 1997/12/05 00:00
PHST- 1997/12/05 00:00 [pubmed]
PHST- 1997/12/05 00:01 [medline]
PHST- 1997/12/05 00:00 [entrez]
PST - ppublish
SO  - Mod Pathol. 1997 Nov;10(11):1164-71.